J Cell Biol
2022 Gradient tracking in mating yeast depends on Bud1 inactivation and actin-independent vesicle delivery 2019 Mechanical stress impairs pheromone signaling via Pkc1-mediated regulation of the MAPK scaffold Ste5 Mating yeast cells use an intrinsic polarity site to assemble a pheromone-gradient tracking machine 2018 Differential GAP requirement for Cdc42-GTP polarization during proliferation and sexual reproduction Inhibition of Ras activity coordinates cell fusion with cell-cell contact during yeast mating 2017 Membrane curvature directs the localization of Cdc42p to novel foci required for cell-cell fusion Spatial and temporal signal processing and decision making by MAPK pathways TORC1 signaling exerts spatial control over microtubule dynamics by promoting nuclear export of Stu2 2015 Pheromone-encoding mRNA is transported to the yeast mating projection by specific RNP granules 2010 Dcp2 phosphorylation by Ste20 modulates stress granule assembly and mRNA decay in Saccharomyces cerevisiae 2009 Antagonistic regulation of Fus2p nuclear localization by pheromone signaling and the cell cycle 2008 Ergosterol promotes pheromone signaling and plasma membrane fusion in mating yeast Dynamic localization of yeast Fus2p to an expanding ring at the cell fusion junction during mating 2007 The Golgi-resident protease Kex2 acts in conjunction with Prm1 to facilitate cell fusion during yeast mating YGR198w (YPP1) targets A30P alpha-synuclein to the vacuole for degradation 2006 Disappearance of the budding yeast Bub2-Bfa1 complex from the mother-bound spindle pole contributes to mitotic exit Bqt2p is essential for initiating telomere clustering upon pheromone sensing in fission yeast Sumoylation of the budding yeast kinetochore protein Ndc10 is required for Ndc10 spindle localization and regulation of anaphase spindle elongation 2005 Spindle-independent condensation-mediated segregation of yeast ribosomal DNA in late anaphase Role of mitochondria in the pheromone- and amiodarone-induced programmed death of yeast 2004 A conserved protein interaction network involving the yeast MAP kinases Fus3 and Kss1 Regulation of polarized growth initiation and termination cycles by the polarisome and Cdc42 regulators "Yeast Miro GTPase, Gem1p, regulates mitochondrial morphology via a novel pathway" Pheromone-induced polarization is dependent on the Fus3p MAPK acting through the formin Bni1p 2003 Cdc48p is required for the cell cycle commitment point at Start via degradation of the G1-CDK inhibitor Far1p Dynamic phosphoregulation of the cortical actin cytoskeleton and endocytic machinery revealed by real-time chemical genetic analysis 2002 The conserved Pkh-Ypk kinase cascade is required for endocytosis in yeast "Sla1p serves as the targeting signal recognition factor for NPFX(1,2)D-mediated endocytosis" The yeast nuclear pore complex functionally interacts with components of the spindle assembly checkpoint 2001 Molecular chaperones in the yeast endoplasmic reticulum maintain the solubility of proteins for retrotranslocation and degradation UGO1 encodes an outer membrane protein required for mitochondrial fusion 2000 Dynamic localization of protein phosphatase type 1 in the mitotic cell cycle of Saccharomyces cerevisiae "Fission yeast myosin-I, Myo1p, stimulates actin assembly by Arp2/3 complex and shares functions with WASp" Nucleocytoplasmic shuttling of the Cdc42p exchange factor Cdc24p The F-box protein Rcy1p is involved in endocytic membrane traffic and recycling out of an early endosome in Saccharomyces cerevisiae Recycling of the yeast a-factor receptor "Prm1p, a pheromone-regulated multispanning membrane protein, facilitates plasma membrane fusion during yeast mating" 1999 The GTP-binding protein Rho1p is required for cell cycle progression and polarization of the yeast cell "Differential regulation of the Kar3p kinesin-related protein by two associated proteins, Cik1p and Vik1p" A Cdc24p-Far1p-Gbetagamma protein complex required for yeast orientation during mating "Chs7p, a new protein involved in the control of protein export from the endoplasmic reticulum that is specifically engaged in the regulation of chitin synthesis in Saccharomyces cerevisiae" "Role for Drs2p, a P-type ATPase and potential aminophospholipid translocase, in yeast late Golgi function" 1998 Cytoplasmic tail phosphorylation of the alpha-factor receptor is required for its ubiquitination and internalization "Iqg1p, a yeast homologue of the mammalian IQGAPs, mediates cdc42p effects on the actin cytoskeleton" "A large PEST-like sequence directs the ubiquitination, endocytosis, and vacuolar degradation of the yeast a-factor receptor" Dual roles for Ste24p in yeast a-factor maturation: NH2-terminal proteolysis and COOH-terminal CAAX processing Rvs161p interacts with Fus2p to promote cell fusion in Saccharomyces cerevisiae A role for a protease in morphogenic responses during yeast cell fusion Pheromone-regulated genes required for yeast mating differentiation Role of the yeast Gin4p protein kinase in septin assembly and the relationship between septin assembly and septin function Reconstitution of retrograde transport from the Golgi to the ER in vitro 1997 KAR5 encodes a novel pheromone-inducible protein required for homotypic nuclear fusion Biogenesis of the Saccharomyces cerevisiae mating pheromone a-factor "A novel membrane-associated metalloprotease, Ste24p, is required for the first step of NH2-terminal processing of the yeast a-factor precursor" Novel genes involved in endosomal traffic in yeast revealed by suppression of a targeting-defective plasma membrane ATPase mutant "SOI1 encodes a novel, conserved protein that promotes TGN-endosomal cycling of Kex2p and other membrane proteins by modulating the function of two TGN localization signals" Targeting of chitin synthase 3 to polarized growth sites in yeast requires Chs5p and Myo2p 1996 Cell fusion during yeast mating requires high levels of a-factor mating pheromone Amino acid permeases require COPII components and the ER resident membrane protein Shr3p for packaging into transport vesicles in vitro "Assembly of ER-associated protein degradation in vitro: dependence on cytosol, calnexin, and ATP" Activation of an MAP kinase cascade leads to Sir3p hyperphosphorylation and strengthens transcriptional silencing Role of the ABC transporter Ste6 in cell fusion during yeast conjugation Ubiquitination of the yeast a-factor receptor The sequence NPFXD defines a new class of endocytosis signal in Saccharomyces cerevisiae Pea2 protein of yeast is localized to sites of polarized growth and is required for efficient mating and bipolar budding "Pds1p, an inhibitor of anaphase in budding yeast, plays a critical role in the APC and checkpoint pathway(s)" 1995 Fus2 localizes near the site of cell fusion and is required for both cell fusion and nuclear alignment during zygote formation "DNM1, a dynamin-related gene, participates in endosomal trafficking in yeast" "Glycosyl phosphatidylinositol-dependent cross-linking of alpha-agglutinin and beta 1,6-glucan in the Saccharomyces cerevisiae cell wall" FAR1 is required for oriented polarization of yeast cells in response to mating pheromones The tyrosine kinase substrate eps15 is constitutively associated with the plasma membrane adaptor AP-2 Saccharomyces cerevisiae cells execute a default pathway to select a mate in the absence of pheromone gradients VPS27 controls vacuolar and endocytic traffic through a prevacuolar compartment in Saccharomyces cerevisiae "Spindle dynamics and cell cycle regulation of dynein in the budding yeast, Saccharomyces cerevisiae" 1994 Endocytosis is required for the growth of vacuolar H(+)-ATPase-defective yeast: identification of six new END genes Localization of the Kar3 kinesin heavy chain-related protein requires the Cik1 interacting protein "Role of three rab5-like GTPases, Ypt51p, Ypt52p, and Ypt53p, in the endocytic and vacuolar protein sorting pathways of yeast" 1993 Cis- and trans-acting functions required for endocytosis of the yeast pheromone receptors The a-factor transporter (STE6 gene product) and cell polarity in the yeast Saccharomyces cerevisiae end3 and end4: two mutants defective in receptor-mediated and fluid-phase endocytosis in Saccharomyces cerevisiae Clathrin facilitates the internalization of seven transmembrane segment receptors for mating pheromones in yeast Reconstitution of protein translocation from solubilized yeast membranes reveals topologically distinct roles for BiP and cytosolic Hsc70 A Sec63p-BiP complex from yeast is required for protein translocation in a reconstituted proteoliposome Yeast vacuolar proenzymes are sorted in the late Golgi complex and transported to the vacuole via a prevacuolar endosome-like compartment 1992 "Effects of null mutations and overexpression of capping protein on morphogenesis, actin distribution and polarized secretion in yeast" Characterization of TPM1 disrupted yeast cells indicates an involvement of tropomyosin in directed vesicular transport Selective and immediate effects of clathrin heavy chain mutations on Golgi membrane protein retention in Saccharomyces cerevisiae 1991 Cellular morphogenesis in the Saccharomyces cerevisiae cell cycle: localization of the CDC3 gene product and the timing of events at the budding site Immunolocalization of Kex2 protease identifies a putative late Golgi compartment in the yeast Saccharomyces cerevisiae The function of chitin synthases 2 and 3 in the Saccharomyces cerevisiae cell cycle MPS1 and MPS2: novel yeast genes defining distinct steps of spindle pole body duplication The Saccharomyces cerevisiae MYO2 gene encodes an essential myosin for vectorial transport of vesicles Sequence of the clathrin heavy chain from Saccharomyces cerevisiae and requirement of the COOH terminus for clathrin function A role for unsaturated fatty acids in mitochondrial movement and inheritance 1990 Isolation of a functional vesicular intermediate that mediates ER to Golgi transport in yeast Identification and initial characterization of an autocrine pheromone receptor in the protozoan ciliate Euplotes raikovi Detection of an intermediate compartment involved in transport of alpha-factor from the plasma membrane to the vacuole in yeast The SPA2 gene of Saccharomyces cerevisiae is important for pheromone-induced morphogenesis and efficient mating 1989 "A novel GTP-binding protein, Sar1p, is involved in transport from the endoplasmic reticulum to the Golgi apparatus" Multiple genes are required for proper insertion of secretory proteins into the endoplasmic reticulum in yeast Secretion in yeast: preprotein binding to a membrane receptor and ATP-dependent translocation are sequential and separable events in vitro 1988 Full-length prepro-alpha-factor can be translocated across the mammalian microsomal membrane only if translation has not terminated Protein transport to the vacuole and receptor-mediated endocytosis by clathrin heavy chain-deficient yeast Reconstitution of protein transport from the endoplasmic reticulum to the Golgi complex in yeast: the acceptor Golgi compartment is defective in the sec23 mutant 1987 Characterization of new mutants in the early part of the yeast secretory pathway isolated by a [3H]mannose suicide selection 1986 Protein translocation across the yeast microsomal membrane is stimulated by a soluble factor Secretory protein translocation in a yeast cell-free system can occur posttranslationally and requires ATP hydrolysis 1985 Defective temporal and spatial control of flagellar assembly in a mutant of Chlamydomonas reinhardtii with variable flagellar number 1984 Bud formation by the yeast Saccharomyces cerevisiae is directly dependent on 'start' Specific early-G1 blocks accompanied with stringent response in Saccharomyces cerevisiae lead to growth arrest in resting state similar to the G0 of higher eucaryotes 1980 Localized secretion of acid phosphatase reflects the pattern of cell surface growth in Saccharomyces cerevisiae Mutants of Saccharomyces cerevisiae unresponsive to cell division control by polypeptide mating hormone Cell cycle phase expansion in nitrogen-limited cultures of Saccharomyces cerevisiae 1976 Functional significance of the variations in the geometrical organization of tight junction networks 1968 Morphological and functional aspects of an insect epidermal gland 1962 Polyester-methacrylate embedments for electron microscopy
