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J Cell Biol

Title:		"Yeast Miro GTPase, Gem1p, regulates mitochondrial morphology via a novel pathway"
Author(s):		Frederick RL; McCaffery JM; Cunningham KW; Okamoto K; Shaw JM;
Address:		"Department of Biology, University of Utah, Salt Lake City, UT 84112, USA"
Journal Title:		J Cell Biol
Year:		2004
Volume:		167
Issue:		1
Page Number:		87 - 98
DOI:		10.1083/jcb.200405100
ISSN/ISBN:		0021-9525 (Print) 1540-8140 (Electronic) 0021-9525 (Linking)
Abstract:		"Cell signaling events elicit changes in mitochondrial shape and activity. However, few mitochondrial proteins that interact with signaling pathways have been identified. Candidates include the conserved mitochondrial Rho (Miro) family of proteins, which contain two GTPase domains flanking a pair of calcium-binding EF-hand motifs. We show that Gem1p (yeast Miro; encoded by YAL048C) is a tail-anchored outer mitochondrial membrane protein. Cells lacking Gem1p contain collapsed, globular, or grape-like mitochondria. We demonstrate that Gem1p is not an essential component of characterized pathways that regulate mitochondrial dynamics. Genetic studies indicate both GTPase domains and EF-hand motifs, which are exposed to the cytoplasm, are required for Gem1p function. Although overexpression of a mutant human Miro protein caused increased apoptotic activity in cultured cells (Fransson et al., 2003. J. Biol. Chem. 278:6495-6502), Gem1p is not required for pheromone-induced yeast cell death. Thus, Gem1p defines a novel mitochondrial morphology pathway which may integrate cell signaling events with mitochondrial dynamics"
Keywords:		"Actins/metabolism Amino Acid Motifs Amino Acid Sequence Apoptosis Cytoplasm/metabolism Cytoskeleton/metabolism DNA, Mitochondrial/metabolism GTP Phosphohydrolases/metabolism Glucose/metabolism Glycerol/metabolism Image Processing, Computer-Assisted Intrac;"
Notes:		"MedlineFrederick, Rebecca L McCaffery, J Michael Cunningham, Kyle W Okamoto, Koji Shaw, Janet M eng R01 GM053466/GM/NIGMS NIH HHS/ 5P30 CA 42014/CA/NCI NIH HHS/ S10 RR019409-01/RR/NCRR NIH HHS/ GM 53466/GM/NIGMS NIH HHS/ P30 CA042014/CA/NCI NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. 2004/10/14 J Cell Biol. 2004 Oct 11; 167(1):87-98. doi: 10.1083/jcb.200405100"