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J Cell Biol

Title:		Protein translocation across the yeast microsomal membrane is stimulated by a soluble factor
Author(s):		Waters MG; Chirico WJ; Blobel G;
Address:
Journal Title:		J Cell Biol
Year:		1986
Volume:		103
Issue:		6 Pt 2
Page Number:		2629 - 2636
DOI:		10.1083/jcb.103.6.2629
ISSN/ISBN:		0021-9525 (Print) 1540-8140 (Electronic) 0021-9525 (Linking)
Abstract:		"We have found that a soluble activity present in the postribosomal supernatant fraction of Saccharomyces cerevisiae stimulates posttranslational translocation of yeast prepro-alpha-factor across yeast microsomal membranes. Stimulation of translocation is not due to a nonspecific affect on ATP levels. The activity is likely to be due to protein(s) as it is destroyed by N-ethylmaleimide, protease, or heat treatment but not by incubation with RNase. Its apparent sedimentation coefficient is approximately 9.6 S"
Keywords:		"Biological Transport, Active Cytoplasm/physiology Endoplasmic Reticulum/*metabolism Fungal Proteins/physiology Mating Factor Microsomes/*metabolism Peptides/*metabolism Protein Precursors/*metabolism Protein Processing, Post-Translational Ribonucleoprotei;"
Notes:		"MedlineWaters, M G Chirico, W J Blobel, G eng GM-07982/GM/NIGMS NIH HHS/ GM-27155/GM/NIGMS NIH HHS/ Research Support, U.S. Gov't, P.H.S. 1986/12/01 J Cell Biol. 1986 Dec; 103(6 Pt 2):2629-36. doi: 10.1083/jcb.103.6.2629"