| Title: | Functional divergence of principal alcohol o-acyltransferase for biosynthesis of volatile acetate esters among tomato wild species (Solanum Sect. Lycopersicon) |
| Author(s): | Kamiyoshihara Y; Miyajima S; Miyagawa Y; Moriyama K; Mizuno S; Goulet C; Klee H; Tateishi A; |
| Address: | "College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, 252-0880, Japan; Graduate School of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, 252-0880, Japan. Electronic address: kamiyoshihara.yuusuke@nihon-u.ac.jp. Graduate School of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, 252-0880, Japan. College of Biological Sciences, University of Tsukuba, Tsukuba, Ibaraki, 305-8575, Japan. College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, 252-0880, Japan; Graduate School of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, 252-0880, Japan. Departement de Phytologie, Universite Laval, Quebec City, Qc, G1V 0A6, Canada. Horticultural Sciences, University of Florida, Gainesville, FL 32611-0690, USA" |
| DOI: | 10.1016/j.plantsci.2020.110612 |
| ISSN/ISBN: | 1873-2259 (Electronic) 0168-9452 (Linking) |
| Abstract: | "Volatile esters are the chemicals that have multiple physiological functions including plant defense responses and reproduction. From a human perspective, the esters largely contribute to the fruity aroma of freshy fruits. Composition of volatile esters show a significant diversity among the wild tomato species (Solanum sect. Lycopersicon). To address the basis for this divergence, here we conducted functional analysis of a gene encoding major alcohol o-acyltransferase (AAT1) that catalyzes volatile ester formation. Although AAT1 transcripts were highly expressed in the ripe fruits of all the wild species examined, their enzymatic properties significantly differed due to amino acid sequence variations. Notably, AAT1s from S. pennellii showed the highest ability to produce acetate esters whereas AAT1s from S. neorickii, S. chmielewskii and S. habrochaites had the lowest activities. Further, screenings using domain-swapped or point-mutated AAT1s allowed us to identify Met/Thr352 as one of the critical residues related to the transferase activity with acetyl-CoA. This finding is potentially applied to aroma engineering in which a site-directed mutagenesis at this position in alcohol o-acyltransferases could enable to manipulate volatile ester levels in ripe fruits" |
| Keywords: | "Acyltransferases/genetics/*metabolism Crops, Agricultural/genetics/metabolism Esters/*metabolism Fruit/genetics/metabolism Gene Expression Regulation, Plant Genes, Plant Genetic Variation Genotype Solanum lycopersicum/*genetics/*metabolism *Odorants Plant;" |
| Notes: | "MedlineKamiyoshihara, Yusuke Miyajima, Sakurako Miyagawa, Yota Moriyama, Kazuki Mizuno, Shinji Goulet, Charles Klee, Harry Tateishi, Akira eng Ireland 2020/11/13 Plant Sci. 2020 Nov; 300:110612. doi: 10.1016/j.plantsci.2020.110612. Epub 2020 Jul 25" |
