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Methods Mol Biol

Title:		Analyses of Plant UDP-Dependent Glycosyltransferases to Identify Their Volatile Substrates Using Recombinant Proteins
Author(s):		Kamiyoshihara Y; Tieman DM; Klee HJ;
Address:		"Horticultural Sciences Department, University of Florida, Building 885, Gainesville, FL, 32611-0690, USA. Horticultural Sciences Department, University of Florida, Building 885, Gainesville, FL, 32611-0690, USA. hjklee@ufl.edu"
Journal Title:		Methods Mol Biol
Year:		2016
Volume:		1363
Issue:
Page Number:		199 - 207
DOI:		10.1007/978-1-4939-3115-6_16
ISSN/ISBN:		1940-6029 (Electronic) 1064-3745 (Linking)
Abstract:		"Glycosylation is one of major modifications for plant secondary metabolites. In the case of volatile compounds, glycosylation makes them nonvolatile and odorless. Identification of UDP-dependent glycosyltransferases responsible for volatile glycosylation is essential to understand the regulatory mechanism of volatile release from plant tissues. Here, we describe an efficient protocol to find possible combinations of volatiles/glycosyltransferases using tomato (Solanum lycopersicum) enzymes expressed in Escherichia coli. The presented method requires a basic gas chromatography system and conventional laboratory tools"
Keywords:		"Chromatography, Gas Glycosides Glycosylation Glycosyltransferases/genetics/*metabolism Hydrolysis Solanum lycopersicum/genetics/metabolism Plants/genetics/*metabolism Recombinant Proteins/metabolism Uridine Diphosphate/*metabolism Volatile Organic Compoun;"
Notes:		"MedlineKamiyoshihara, Yusuke Tieman, Denise M Klee, Harry J eng Research Support, U.S. Gov't, Non-P.H.S. 2015/11/19 Methods Mol Biol. 2016; 1363:199-207. doi: 10.1007/978-1-4939-3115-6_16"