| Title: | The protein kinase homologue Ste20p is required to link the yeast pheromone response G-protein beta gamma subunits to downstream signalling components |
| Author(s): | Leberer E; Dignard D; Harcus D; Thomas DY; Whiteway M; |
| Address: | "Biotechnology Research Institute, National Research Council of Canada" |
| DOI: | 10.1002/j.1460-2075.1992.tb05587.x |
| ISSN/ISBN: | 0261-4189 (Print) 1460-2075 (Electronic) 0261-4189 (Linking) |
| Abstract: | "In the yeast Saccharomyces cerevisiae the G-protein beta gamma subunits have been shown to trigger downstream events of the pheromone response pathway. We have identified a new gene, designated STE20, which encodes a protein kinase homologue with sequence similarity to protein kinase C, which is required to transmit the pheromone signal from G beta gamma to downstream components of the signalling pathway. Overproduction of the kinase suppresses the mating defect of dominant-negative G beta mutations providing genetic evidence for an interaction with G beta, and epistasis experiments show that this kinase functions after or at the same point as G beta gamma, but before any of the other currently identified components of the signalling pathway. This points to a potentially new mechanism of G-protein mediated signal transduction, the activation of a protein kinase through G beta gamma" |
| Keywords: | "Amino Acid Sequence Base Sequence Blotting, Northern Chromosome Mapping DNA, Fungal/metabolism Fungal Proteins/genetics/*metabolism GTP-Binding Proteins/*metabolism Intracellular Signaling Peptides and Proteins MAP Kinase Kinase Kinases Molecular Sequence;" |
| Notes: | "MedlineLeberer, E Dignard, D Harcus, D Thomas, D Y Whiteway, M eng Research Support, Non-U.S. Gov't England 1992/12/01 EMBO J. 1992 Dec; 11(13):4815-24. doi: 10.1002/j.1460-2075.1992.tb05587.x" |
