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Science

Title:		Direct inhibition of the yeast cyclin-dependent kinase Cdc28-Cln by Far1
Author(s):		Peter M; Herskowitz I;
Address:		"Department of Biochemistry and Biophysics, University of California at San Francisco 94143-0448"
Journal Title:		Science
Year:		1994
Volume:		265
Issue:		5176
Page Number:		1228 - 1231
DOI:		10.1126/science.8066461
ISSN/ISBN:		0036-8075 (Print) 0036-8075 (Linking)
Abstract:		"Cell cycle arrest of Saccharomyces cerevisiae in G1 by the antimitogen alpha-factor is mediated by activation of a signal transduction pathway that results in inhibition of the cyclin-dependent kinase Cdc28-Cln. The Far1 protein is required for cell cycle arrest and associates with the Cdc28-Cln complex. The kinase activity of Cdc28-Cln was directly inhibited by Far1 both in vivo and in vitro, thus demonstrating that Far1 acts at the final step in the alpha-factor response pathway by inhibiting a G1 cyclin-dependent kinase"
Keywords:		"CDC28 Protein Kinase, S cerevisiae/*antagonists & inhibitors/metabolism *Cell Cycle Proteins Cyclin-Dependent Kinase Inhibitor Proteins Cyclins/genetics/*metabolism Fungal Proteins/metabolism/*pharmacology G1 Phase Mating Factor Peptides/pharmacology Phos;"
Notes:		"MedlinePeter, M Herskowitz, I eng GM31286/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1994/08/26 Science. 1994 Aug 26; 265(5176):1228-31. doi: 10.1126/science.8066461"