| Title: | FAR1 links the signal transduction pathway to the cell cycle machinery in yeast |
| Author(s): | Peter M; Gartner A; Horecka J; Ammerer G; Herskowitz I; |
| Address: | "Department of Biochemistry and Biophysics, University of California, San Francisco 94143-0448" |
| DOI: | 10.1016/0092-8674(93)90254-n |
| ISSN/ISBN: | 0092-8674 (Print) 0092-8674 (Linking) |
| Abstract: | "Alpha factor induces arrest of yeast a cells in G1 and transcription of genes involved in mating. Prior work indicates that FUS3, a member of the MAP kinase family, and FAR1, whose molecular activity is unknown, contribute to cell cycle arrest by inhibiting G1 cyclins. Here we show that FAR1 is a substrate for FUS3 and that this phosphorylation regulates association of FAR1 with CDC28-CLN2 kinase. We show also that FAR1 is phosphorylated in vitro by the CDC28-CLN2 complex and in vivo in a CDC28-dependent manner. Mutational analysis of FAR1 reveals a correlation between its ability to associate with CDC28-CLN2 and to arrest the cell cycle. These results suggest that FAR1 protein is the link between the signaling pathway and the cell cycle machinery" |
| Keywords: | "Amino Acid Sequence Base Sequence CDC28 Protein Kinase, S cerevisiae/metabolism Cell Cycle/*physiology *Cell Cycle Proteins Cyclin-Dependent Kinase Inhibitor Proteins DNA Mutational Analysis Fungal Proteins/*metabolism Mating Factor *Mitogen-Activated Pro;" |
| Notes: | "MedlinePeter, M Gartner, A Horecka, J Ammerer, G Herskowitz, I eng GM30027/GM/NIGMS NIH HHS/ GM31286/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1993/05/21 Cell. 1993 May 21; 73(4):747-60. doi: 10.1016/0092-8674(93)90254-n" |
