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Phytochem Anal

Title:		Screening of plant extracts for antioxidant activity: a comparative study on three testing methods
Author(s):		Koleva II; van Beek TA; Linssen JP; de Groot A; Evstatieva LN;
Address:		"Laboratory of Organic Chemistry, Wageningen University, Dreijenplein 8, 6703 HB Wageningen, The Netherlands"
Journal Title:		Phytochem Anal
Year:		2002
Volume:		13
Issue:		1
Page Number:		8 - 17
DOI:		10.1002/pca.611
ISSN/ISBN:		0958-0344 (Print) 0958-0344 (Linking)
Abstract:		"Three methods widely employed in the evaluation of antioxidant activity, namely 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method, static headspace gas chromatography (HS-GC) and beta-carotene bleaching test (BCBT), have been compared with regard to their application in the screening of plant extracts. The strengths and limitations of each method have been illustrated by testing a number of extracts, of differing polarity, from plants of the genus Sideritis, and two known antioxidants (butylated hydroxytoluene and rosmarinic acid). The sample polarity was important for the exhibited activity in the BCBT and HS-GC methods but not for the DPPH method. The complex composition of the extracts and partition phenomena affected their activity in each assay. The value of the BCBT method appears to be limited to less polar samples. Although slow, the HS-GC method is preferable for assessing the antioxidant inhibitory properties on the formation of unwanted secondary volatile products. Being rapid, simple and independent of sample polarity, the DPPH method is very convenient for the quick screening of many samples for radical scavenging activity"
Keywords:		"Antioxidants/*isolation & purification/metabolism Biphenyl Compounds Chromatography, Gas/*methods Free Radical Scavengers/metabolism Free Radicals/*metabolism Lamiaceae/*chemistry Picrates Plant Extracts/*chemistry Plants, Medicinal/chemistry Reference St;"
Notes:		"MedlineKoleva, Irina I van Beek, Teris A Linssen, Jozef P H de Groot, Aede Evstatieva, Lyuba N eng Comparative Study Research Support, Non-U.S. Gov't England 2002/03/20 Phytochem Anal. 2002 Jan-Feb; 13(1):8-17. doi: 10.1002/pca.611"