| Title: | The glucosinolate breakdown product indole-3-carbinol acts as an auxin antagonist in roots of Arabidopsis thaliana |
| Author(s): | Katz E; Nisani S; Yadav BS; Woldemariam MG; Shai B; Obolski U; Ehrlich M; Shani E; Jander G; Chamovitz DA; |
| Address: | "Molecular Biology and Ecology of Plants, Tel Aviv University, Ramat Aviv, 69978, Israel. Boyce Thompson Institute, Ithaca, NY, 14853, USA. Cell Research and Immunology, Tel Aviv University, Ramat Aviv, 69978, Israel" |
| ISSN/ISBN: | 1365-313X (Electronic) 0960-7412 (Linking) |
| Abstract: | "The glucosinolate breakdown product indole-3-carbinol functions in cruciferous vegetables as a protective agent against foraging insects. While the toxic and deterrent effects of glucosinolate breakdown on herbivores and pathogens have been studied extensively, the secondary responses that are induced in the plant by indole-3-carbinol remain relatively uninvestigated. Here we examined the hypothesis that indole-3-carbinol plays a role in influencing plant growth and development by manipulating auxin signaling. We show that indole-3-carbinol rapidly and reversibly inhibits root elongation in a dose-dependent manner, and that this inhibition is accompanied by a loss of auxin activity in the root meristem. A direct interaction between indole-3-carbinol and the auxin perception machinery was suggested, as application of indole-3-carbinol rescues auxin-induced root phenotypes. In vitro and yeast-based protein interaction studies showed that indole-3-carbinol perturbs the auxin-dependent interaction of Transport Inhibitor Response (TIR1) with auxin/3-indoleacetic acid (Aux/IAAs) proteins, further supporting the possibility that indole-3-carbinol acts as an auxin antagonist. The results indicate that chemicals whose production is induced by herbivory, such as indole-3-carbinol, function not only to repel herbivores, but also as signaling molecules that directly compete with auxin to fine tune plant growth and development" |
| Keywords: | "Arabidopsis/*drug effects/*metabolism Arabidopsis Proteins/metabolism Gene Expression Regulation, Plant/drug effects Glucosinolates/*metabolism Indoleacetic Acids/antagonists & inhibitors Indoles/*metabolism/*pharmacology Plant Roots/*drug effects/*metabo;" |
| Notes: | "MedlineKatz, Ella Nisani, Sophia Yadav, Brijesh S Woldemariam, Melkamu G Shai, Ben Obolski, Uri Ehrlich, Marcelo Shani, Eilon Jander, Georg Chamovitz, Daniel A eng Research Support, Non-U.S. Gov't England 2015/03/12 Plant J. 2015 May; 82(4):547-55. doi: 10.1111/tpj.12824. Epub 2015 Apr 13" |
