| Title: | "The genetic structure and diversity of the A and B mating-type genes from the tropical oyster mushroom, Pleurotus djamor" |
| Author(s): | James TY; Liou SR; Vilgalys R; |
| Address: | "Department of Biology, Box 90338, Duke University, Durham, NC 27708, USA. tyj2@duke.edu" |
| DOI: | 10.1016/j.fgb.2004.04.005 |
| ISSN/ISBN: | 1087-1845 (Print) 1087-1845 (Linking) |
| Abstract: | "In most heterothallic mushroom species, inbreeding is avoided by an incompatibility system determined by two loci each with multiple alleles (the A and B mating-type loci). In this study we investigated the genetic structure of the mating-type loci in the tropical oyster mushroom Pleurotus djamor using both positional cloning and degenerate PCR methods. DNA sequences from genomic regions cosegregating with the mating-type loci of P. djamor revealed homeodomain transcription factors (A) and pheromone receptors (B), suggesting the genetic basis for mating-type determination in P. djamor is the same as in the model mushroom species. Three pheromone receptors were detected in a single homokaryotic isolate of P. djamor. Only one pair of homeodomain genes was detected in the A mating-type region. It is hypothesized that the A mating-type locus of P. djamor is comprised of only one homeodomain pair, which may explain the lower number of A mating-type alleles relative to other mushroom species" |
| Keywords: | "Amino Acid Sequence Chromosome Mapping Gene Library *Genes, Fungal *Genes, Mating Type, Fungal *Genetic Variation Molecular Sequence Data Phylogeny Pleurotus/classification/*genetics Receptors, Pheromone/genetics Sequence Homology, Amino Acid Transcriptio;" |
| Notes: | "MedlineJames, Timothy Y Liou, Shian-Ren Vilgalys, Rytas eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. 2004/06/29 Fungal Genet Biol. 2004 Aug; 41(8):813-25. doi: 10.1016/j.fgb.2004.04.005" |
