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Fungal Genet Biol

Title:		Evolution of the gene encoding mitochondrial intermediate peptidase and its cosegregation with the A mating-type locus of mushroom fungi
Author(s):		James TY; Kues U; Rehner SA; Vilgalys R;
Address:		"Department of Biology, Box 90338, Duke University, Durham, NC 27708, USA. tyj2@duke.edu"
Journal Title:		Fungal Genet Biol
Year:		2004
Volume:		41
Issue:		3
Page Number:		381 - 390
DOI:		10.1016/j.fgb.2003.11.008
ISSN/ISBN:		1087-1845 (Print) 1087-1845 (Linking)
Abstract:		"The high level of DNA polymorphism at the mating-type loci of mushroom fungi has made the cloning of mating-type genes difficult. As an alternative to strategies that employ sequence conservation, an approach utilizing conserved gene order could facilitate the cloning of A mating-type genes from mushroom fungi. It has been shown that a gene encoding a mitochondrial intermediate peptidase (MIP) is very close ( < 1 kbp) to the A mating-type locus of two model mushroom species. In this study, the cosegregation of MIP and the A mating-type locus was studied by genotyping progeny of seven additional mushroom species using PCR and genetic crosses. No evidence of any recombination between MIP and the A mating-type locus was detected among all seven species. Phylogenetic analysis of MIP sequences from diverse mushroom species agrees with the current organismal phylogeny, suggesting the sequences are generally orthologous"
Keywords:		"Agaricales/classification/*enzymology/*genetics Base Sequence DNA Primers DNA, Fungal/genetics/isolation & purification Genetic Variation Mating Factor Metalloendopeptidases/*genetics Peptides/*genetics Phylogeny;"
Notes:		"MedlineJames, Timothy Y Kues, Ursula Rehner, Stephen A Vilgalys, Rytas eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. 2004/02/06 Fungal Genet Biol. 2004 Mar; 41(3):381-90. doi: 10.1016/j.fgb.2003.11.008"