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Molecules

Title:		"Volatile Organic Compounds, Bacterial Airway Microbiome, Spirometry and Exercise Performance of Patients after Surgical Repair of Congenital Diaphragmatic Hernia"
Author(s):		Warncke G; Singer G; Windhaber J; Schabl L; Friehs E; Miekisch W; Gierschner P; Klymiuk I; Eber E; Zeder K; Pfleger A; Obermuller B; Till H; Castellani C;
Address:		"Department of Paediatric and Adolescent Surgery, Medical University Graz, 8036 Graz, Austria. Department of Anesthesiology and Intensive Care Medicine, Rostock Medical Breath Research Analytics and Technologies (ROMBAT), Rostock University Medical Centre, 18057 Rostock, Germany. Core Facility Molecular Biology, Center for Medical Research, Medical University of Graz, 8036 Graz, Austria. Department of Paediatrics and Adolescent Medicine, Division of Paediatric Pulmonology and Allergology, Medical University of Graz, 8036 Graz, Austria"
Journal Title:		Molecules
Year:		2021
Volume:		20210126
Issue:		3
Page Number:		-
DOI:		10.3390/molecules26030645
ISSN/ISBN:		1420-3049 (Electronic) 1420-3049 (Linking)
Abstract:		"The aim of this study was to analyze the exhaled volatile organic compounds (VOCs) profile, airway microbiome, lung function and exercise performance in congenital diaphragmatic hernia (CDH) patients compared to healthy age and sex-matched controls. A total of nine patients (median age 9 years, range 6-13 years) treated for CDH were included. Exhaled VOCs were measured by GC-MS. Airway microbiome was determined from deep induced sputum by 16S rRNA gene sequencing. Patients underwent conventional spirometry and exhausting bicycle spiroergometry. The exhaled VOC profile showed significantly higher levels of cyclohexane and significantly lower levels of acetone and 2-methylbutane in CDH patients. Microbiome analysis revealed no significant differences for alpha-diversity, beta-diversity and LefSe analysis. CDH patients had significantly lower relative abundances of Pasteurellales and Pasteurellaceae. CDH patients exhibited a significantly reduced Tiffeneau Index. Spiroergometry showed no significant differences. This is the first study to report the VOCs profile and airway microbiome in patients with CDH. Elevations of cyclohexane observed in the CDH group have also been reported in cases of lung cancer and pneumonia. CDH patients had no signs of impaired physical performance capacity, fueling controversial reports in the literature"
Keywords:		"Acetone/analysis Adolescent Bacteria/*classification/genetics/isolation & purification Child DNA, Bacterial/genetics DNA, Ribosomal/genetics Exercise Female Hernias, Diaphragmatic, Congenital/metabolism/physiopathology/*surgery Herniorrhaphy/*methods Huma;"
Notes:		"MedlineWarncke, Gert Singer, Georg Windhaber, Jana Schabl, Lukas Friehs, Elena Miekisch, Wolfram Gierschner, Peter Klymiuk, Ingeborg Eber, Ernst Zeder, Katarina Pfleger, Andreas Obermuller, Beate Till, Holger Castellani, Christoph eng NA/City of Graz/ NA/inVITA - Gesellschaft zur Foerderung der Gesundheit unserer Kinder/ NA/Verein fuer Kinderchirurgie an der Medizinischen Universitaet Graz/ Switzerland 2021/02/04 Molecules. 2021 Jan 26; 26(3):645. doi: 10.3390/molecules26030645"