| Title: | "The role of chemosensory protein 10 in the detection of behaviorally active compounds in brown planthopper, Nilaparvata lugens" |
| Author(s): | Waris MI; Younas A; Adeel MM; Duan SG; Quershi SR; Kaleem Ullah RM; Wang MQ; |
| Address: | "Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China. College of informatics, Huazhong Agricultural University, Wuhan, China" |
| ISSN/ISBN: | 1744-7917 (Electronic) 1672-9609 (Linking) |
| Abstract: | "Chemosensory proteins (CSPs) play important roles in insects' chemoreception, although their specific functional roles have not been fully elucidated. In this study, we conducted the developmental expression patterns and competitive binding assay as well as knock-down assay by RNA interference both in vitro and in vivo to reveal the function of NlugCSP10 from the brown planthopper (BPH), Nilaparvata lugens (Stal), a major pest in rice plants. The results showed that NlugCSP10 messenger RNA was significantly higher in males than in females and correlated to gender, development and wing forms. The fluorescence binding assays revealed that NlugCSP10 exhibited the highest binding affinity with cis-3-hexenyl acetate, eicosane, and (+)-beta-pinene. Behavioral assay revealed that eicosane displayed attractant activity, while cis-3-hexenyl acetate, similar to (+)-beta-pinene significantly repelled N. lugens adults. Silencing of NlugCSP10, which is responsible for cis-3-hexenyl acetate binding, significantly disrupted cis-3-hexenyl acetate communication. Overall, findings of the present study showed that NlugCSP10 could selectively interrelate with numerous volatiles emitted from host plants and these ligands could be designated to develop slow-release mediators that attract/repel N. lugens and subsequently improve the exploration of plans to control this insect pest" |
| Keywords: | "Alkanes/*metabolism Animals Behavior Crops, Agricultural Female Gene Expression Profiling Hemiptera/genetics/*physiology Insect Proteins/genetics/metabolism Insect Repellents/metabolism Male Oryza Pest Control/methods RNA Interference Receptors, Odorant/g;" |
| Notes: | "MedlineWaris, Muhammad Irfan Younas, Aneela Adeel, Muhammad Muzammal Duan, Shuang-Gang Quershi, Sundas Rana Kaleem Ullah, Rana Muhammad Wang, Man-Qun eng 201403030/Special Fund for Agro-scientific Research in the Public Interest of China/ 2017YFD0200400/National Key Research and Development Program of China/ 2016ABA093/Special Technical Innovation of Hubei Province/ Australia 2018/12/30 Insect Sci. 2020 Jun; 27(3):531-544. doi: 10.1111/1744-7917.12659. Epub 2019 Jan 31" |
