| Title: | The terpene synthase gene family in Gossypium hirsutum harbors a linalool synthase GhTPS12 implicated in direct defence responses against herbivores |
| Author(s): | Huang XZ; Xiao YT; Kollner TG; Jing WX; Kou JF; Chen JY; Liu DF; Gu SH; Wu JX; Zhang YJ; Guo YY; |
| Address: | "State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China. Max Planck Institute for Chemical Ecology, Jena, Germany. Institute of Agro-food Science and Technology, Chinese Academy of Agriculture Sciences, Beijing, 100193, China. College of Plant Protection, Northwest A & F University, Yangling, 712100, Shaanxi, China" |
| ISSN/ISBN: | 1365-3040 (Electronic) 0140-7791 (Linking) |
| Abstract: | "Herbivore-induced terpenes have been reported to function as ecological signals in plant-insect interactions. Here, we showed that insect-induced cotton volatile blends contained 16 terpenoid compounds with a relatively high level of linalool. The high diversity of terpene production is derived from a large terpene synthase (TPS) gene family. The TPS gene family of Gossypium hirsutum and Gossypium raimondii consist of 46 and 41 members, respectively. Twelve TPS genes (GhTPS4-15) could be isolated, and protein expression in Escherichia coli revealed catalytic activity for eight GhTPS. The upregulation of the majority of these eight genes additionally supports the function of these genes in herbivore-induced volatile biosynthesis. Furthermore, transgenic Nicotiana tabacum plants overexpressing GhTPS12 were generated, which produced relatively large amounts of (3S)-linalool. In choice tests, female adults of Helicoverpa armigera laid fewer eggs on transgenic plants compared with non-transformed controls. Meanwhile, Myzus persicae preferred feeding on wild-type leaves over leaves of transgenic plants. Our findings demonstrate that transcript accumulation of multiple TPS genes is mainly responsible for the production and diversity of herbivore-induced volatile terpenes in cotton. Also, these genes might play roles in plant defence, in particular, direct defence responses against herbivores" |
| Keywords: | "Acyclic Monoterpenes Alkyl and Aryl Transferases/*genetics/metabolism Animals Aphids Gas Chromatography-Mass Spectrometry Gene Expression Regulation, Plant Gossypium/enzymology/*genetics/*immunology/parasitology Herbivory/*physiology Hydro-Lyases/*metabol;" |
| Notes: | "MedlineHuang, Xin-Zheng Xiao, Yu-Tao Kollner, Tobias G Jing, Wei-Xia Kou, Jun-Feng Chen, Jie-Yin Liu, Dan-Feng Gu, Shao-Hua Wu, Jun-Xiang Zhang, Yong-Jun Guo, Yu-Yuan eng 2017/10/19 Plant Cell Environ. 2018 Jan; 41(1):261-274. doi: 10.1111/pce.13088. Epub 2017 Nov 16" |
