| Title: | Tissue damage and cytotoxic effects of Tagetes minuta essential oil against Lucilia cuprina |
| Author(s): | Chaaban A; Santos V; Martins CEN; Brum JS; Bertoldi FC; Molento MB; |
| Address: | "Laboratory of Parasitic Diseases, Department of Veterinary Sciences, Federal University of Parana, Curitiba, PR, Brazil; Department of Veterinary Medicine, Catarinense Federal Institute, Araquari, SC, Brazil. Electronic address: amanda.chaaban@ifc.edu.br. Department of Veterinary Medicine, Catarinense Federal Institute, Araquari, SC, Brazil. Laboratory of Veterinary Pathology, Department of Veterinary Sciences, Federal University of Parana, Curitiba, PR, Brazil. Agricultural Research and Extension of Santa Catarina, EPAGRI, Itajai, SC, Brazil. Laboratory of Parasitic Diseases, Department of Veterinary Sciences, Federal University of Parana, Curitiba, PR, Brazil; National Institute of Science and Technology, INCT-Livestock, Belo Horizonte, MG, Brazil" |
| DOI: | 10.1016/j.exppara.2019.01.013 |
| ISSN/ISBN: | 1090-2449 (Electronic) 0014-4894 (Linking) |
| Abstract: | "The blowfly Lucilia cuprina has great medico-sanitary and veterinary importance due to the ability of its larval form to develop in decaying organic matter, parasitizing vertebrates. Fly eradication is challenging and the essential oil (EO) of Tagetes minuta (TMEO) have been reported to have therapeutic properties. This study aimed to determine the activity of EO from the aerial parts of T. minuta against third instar larvae (L3) of L. cuprina. Groups of 20 L3 were placed on filter paper, which were impregnated with varying concentrations (0.19; 0.39; 0.79; 1.59; 2.38; 3.18; 4.77; and 6.36?ª+muL/cm(2)) of TMEO solubilized in acetone, ethanol or Tween 20. Histological tissue damage of TMEO was measured in L3 after 24, 48 and 96?ª+h of exposure. Dihydrotagetone (67.64%), trans-ocimene (16.23%), trans-tagetone (10.14%) and verbenone (2.98%) were obtained as major compounds of TMEO. Lethal concentrations of 50%, 24 and 48?ª+h after TMEO exposure were 1.02 and 0.73?ª+muL/cm(2) for acetone; 3.37 and 1.75?ª+muL/cm(2) for ethanol; and 7.46 and 6.11?ª+muL/cm(2) for Tween 20, respectively. TMEO had a significant L3 mortality of 96.6% in acetone, 48?ª+h after contact. Cuticle abnormalities were observed, as well as the loss of digestive tract architecture and vacuolization in fat bodies. TMEO presented time and concentration-dependent effects against L. cuprina. As our study demonstrated a strong insecticide activity of TMEO, we consider that it could be developed into an ecofriendly product against L. cuprina" |
| Keywords: | "Animals Cattle Diptera/*drug effects/growth & development Dose-Response Relationship, Drug Ecosystem Female Gas Chromatography-Mass Spectrometry Larva/drug effects Lethal Dose 50 Male Meat/parasitology Myiasis/drug therapy/prevention & control Oils, Volat;" |
| Notes: | "MedlineChaaban, Amanda Santos, Vera Maria Carvalho Silva Martins, Carlos Eduardo Nogueira Brum, Juliana Sperotto Bertoldi, Fabiano Cleber Molento, Marcelo Beltrao eng 2019/02/06 Exp Parasitol. 2019 Mar; 198:46-52. doi: 10.1016/j.exppara.2019.01.013. Epub 2019 Feb 2" |
