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Insect Sci

Title:		Suppression of pheromone biosynthesis and mating behavior by RNA interference of pheromone gland-specific fatty acyl reductase in Maruca vitrata
Author(s):		Cha WH; Lee DW;
Address:		"Department of Biosafety, Kyungsung University, Busan, Republic of Korea. Metabolomics Research Center for Functional Materials, Kyungsung University, Busan, Republic of Korea"
Journal Title:		Insect Sci
Year:		2022
Volume:		20220125
Issue:		4
Page Number:		1135 - 1144
DOI:		10.1111/1744-7917.12999
ISSN/ISBN:		1744-7917 (Electronic) 1672-9609 (Linking)
Abstract:		"In moths, various enzymes, such as fatty acid synthases, fatty acyl desaturases, and fatty acyl reductases (FARs), are involved in pheromone biosynthesis. In particular, pheromone gland-specific FAR (pgFAR) plays an important role in converting the functional group from carboxylic to alcohol during pheromone biosynthesis. A novel pgFAR of Maruca vitrata, Mvi-pgFAR, was identified through transcriptome sequencing of its pheromone gland. To investigate the involvement of Mvi-pgFAR in pheromone biosynthesis, Mvi-pgFAR was cloned from the pheromone gland and suppressed by RNA interference (RNAi). Mvi-pgFAR harbored several conserved motifs related to NAD(P)H-binding, N-glycosylation, and adenosine / guanosine triphosphate binding. Phylogenetic analysis revealed that Mvi-pgFAR with other lepidopteran pgFARs formed an independent clade. Mvi-pgFAR was specifically expressed only in the pheromone gland. Quantitative real-time polymerase chain reaction showed that the diurnal expression levels of Mvi-pgFAR in the pheromone gland were the highest at 2 h before the scotophase. After primarily confirming Mvi-pgFAR suppression by RNAi, (E,E)-10,12-hexadecadienal (E10E12-16:Ald), a major sex pheromone component, was quantified by gas chromatography. When Mvi-pgFAR was successfully suppressed, E10E12-16:Ald production was reduced by up to half of that of the control, and the mating rate was subsequently decreased. Our results demonstrate that Mvi-pgFAR downregulation can suppress mating behavior by changing the relative sex pheromone component ratio, suggesting that Mvi-pgFAR can be used as a novel control target"
Keywords:		"Amino Acid Sequence Animals *Moths/physiology Oxidoreductases/genetics/metabolism Phylogeny RNA Interference *Sex Attractants/chemistry (E, E)-10, 12-hexadecadienal Maruca vitrata RNAi gas chromatography pgFAR sex pheromone;"
Notes:		"MedlineCha, Wook Hyun Lee, Dae-Weon eng Australia 2022/01/01 Insect Sci. 2022 Aug; 29(4):1135-1144. doi: 10.1111/1744-7917.12999. Epub 2022 Jan 25"