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Food Microbiol

Title:		Detection of Escherichia coli via VOC profiling using secondary electrospray ionization-mass spectrometry (SESI-MS)
Author(s):		Zhu J; Hill JE;
Address:		"University of Vermont, School of Engineering, 33 Colchester Avenue, Burlington, VT 05405-0156, USA"
Journal Title:		Food Microbiol
Year:		2013
Volume:		20130105
Issue:		2
Page Number:		412 - 417
DOI:		10.1016/j.fm.2012.12.008
ISSN/ISBN:		1095-9998 (Electronic) 0740-0020 (Print) 0740-0020 (Linking)
Abstract:		"Escherichia coli O157:H7 (EC O157:H7), as well as its recently emerging non-O157 relatives, are a notorious group of pathogenic bacteria associated with foodborne outbreaks. In this study, we demonstrated that secondary electrospray ionization mass spectrometry (SESI-MS) could be a rapid and accurate detection technology for foodborne pathogens. With SESI-MS volatile organic compound (VOC) profiling, we were able to detect and separate a group of eleven E. coli strains from two major foodborne bacteria, Staphylococcus aureus and Salmonella Typhimurium in three food modeling media. In addition, heatmap analysis of relative peak intensity show that there are six core peaks (m/z of 65, 91, 92, 117, 118 and 119) present and at a similar intensity in all eleven E. coli strains at the experimental conditions we tested. These peaks can be considered conserved VOC biomarkers for E. coli species (robustly produced after just 4 h of growth). Bacterial strain-level differentiation was also attempted via VOC profiling, and we found that EC O157:H7 and EC O145 were differentiable from all other EC strains under the conditions investigated"
Keywords:		"Bacterial Typing Techniques/*methods Escherichia coli/*chemistry/isolation & purification/*metabolism Salmonella typhimurium/chemistry/isolation & purification/metabolism Spectrometry, Mass, Electrospray Ionization/*methods Staphylococcus aureus/chemistry;"
Notes:		"MedlineZhu, Jiangjiang Hill, Jane E eng P20 GM103496/GM/NIGMS NIH HHS/ P20 RR021905/RR/NCRR NIH HHS/ 8 P20 GM103496-07/GM/NIGMS NIH HHS/ 5P20RR021905-07/RR/NCRR NIH HHS/ Evaluation Study Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. England 2013/04/02 Food Microbiol. 2013 Jun; 34(2):412-7. doi: 10.1016/j.fm.2012.12.008. Epub 2013 Jan 5"