« Previous AbstractFast detection of volatile organic compounds from bacterial cultures by secondary electrospray ionization-mass spectrometry    Next AbstractDetection of Escherichia coli via VOC profiling using secondary electrospray ionization-mass spectrometry (SESI-MS) »

J Breath Res

Title:		Detecting bacterial lung infections: in vivo evaluation of in vitro volatile fingerprints
Author(s):		Zhu J; Bean HD; Wargo MJ; Leclair LW; Hill JE;
Address:		"School of Engineering, University of Vermont, Burlington, VT 05405, USA"
Journal Title:		J Breath Res
Year:		2013
Volume:		20130110
Issue:		1
Page Number:		16003 -
DOI:		10.1088/1752-7155/7/1/016003
ISSN/ISBN:		1752-7163 (Electronic) 1752-7155 (Print) 1752-7155 (Linking)
Abstract:		"The identification of bacteria by their volatilomes is of interest to many scientists and clinicians as it holds the promise of diagnosing infections in situ, particularly lung infections via breath analysis. While there are many studies reporting various bacterial volatile biomarkers or fingerprints using in vitro experiments, it has proven difficult to translate these data to in vivo breath analyses. Therefore, we aimed to create secondary electrospray ionization-mass spectrometry (SESI-MS) pathogen fingerprints directly from the breath of mice with lung infections. In this study we demonstrated that SESI-MS is capable of differentiating infected versus uninfected mice, P. aeruginosa-infected versus S. aureus-infected mice, as well as distinguish between infections caused by P. aeruginosa strains PAO1 versus FRD1, with statistical significance (p < 0.05). In addition, we compared in vitro and in vivo volatiles and observed that only 25-34% of peaks are shared between the in vitro and in vivo SESI-MS fingerprints. To the best of our knowledge, these are the first breath volatiles measured for P. aeruginosa PAO1, FRD1, and S. aureus RN450, and the first comparison of in vivo and in vitro volatile profiles from the same strains using the murine infection model"
Keywords:		"Animals Biomarkers/metabolism *Breath Tests Male Mice Mice, Inbred C57BL Pseudomonas Infections/diagnosis Pseudomonas aeruginosa/*metabolism Respiratory Tract Infections/*microbiology Spectrometry, Mass, Electrospray Ionization Staphylococcal Infections/d;"
Notes:		"MedlineZhu, Jiangjiang Bean, Heather D Wargo, Matthew J Leclair, Laurie W Hill, Jane E eng P20 GM103496/GM/NIGMS NIH HHS/ P20 RR021905/RR/NCRR NIH HHS/ 8 P20 GM103496-07/GM/NIGMS NIH HHS/ 5P20RR021905-07/RR/NCRR NIH HHS/ Research Support, N.I.H., Extramural England 2013/01/12 J Breath Res. 2013 Mar; 7(1):016003. doi: 10.1088/1752-7155/7/1/016003. Epub 2013 Jan 10"