| Title: | The identification of an age- and female-specific putative PBAN membrane-receptor protein in pheromone glands of Helicoverpa armigera: possible up-regulation by Juvenile Hormone |
| Author(s): | Rafaeli A; Zakharova T; Lapsker Z; Jurenka RA; |
| Address: | "Institute for Technology & Storage of Agricultural Products, ARO, Volcani Center, PO Box 6, Bet Dagan 50250, Israel. vtada@volcani.agri.gov.il" |
| DOI: | 10.1016/s0965-1748(02)00264-3 |
| ISSN/ISBN: | 0965-1748 (Print) 0965-1748 (Linking) |
| Abstract: | "The present study was designed to determine the age and female specificity of a membrane protein that binds to a pheromone biosynthesis activating neuropeptide (PBAN) ligand and to elucidate the effect of Juvenile Hormone (JH) on binding as well as pheromone activation. The precise age at which developing adult females of Helicoverpa armigera begin to respond to PBAN was determined. PBAN activates in vitro pheromone biosynthesis as well as its intracellular second messenger, cAMP, only in intersegments of newly emerged adult female pheromone glands (i.e. 1-day-old females). An increase in response was observed in 2-day-old females. Intersegments of female pupae and the homologous tissues of adult males do not respond to PBAN. However, in the presence of Juvenile Hormone II (JH II) PBAN induced a response in females, 1 day before emergence (pharate females), but not in younger female pupae. This phenomenon was also observed after topical applications of the JH analog fenoxycarb (FX). In addition the response to PBAN by intersegments of FX-treated emerged adults increased significantly to the level of 2-day-old females. JH II also stimulated the level of incorporation of (35)S-labelled amino acids in female pupae into membrane proteins that are typical in adult intersegments. Using a photoaffinity-biotin labelled PBAN analog we demonstrate specific binding of a membrane protein (estimated MW: 50 kD) in adult females. This binding was not detected in female pupae 3 days before emergence. However, in such female pupae specific binding of the 50 kD protein by the photoaffinity-biotin labelled PBAN analog was induced after JH II or FX treatments thereby providing evidence that JH may up-regulate this putative receptor protein" |
| Keywords: | "Animals Blotting, Western Electrophoresis, Polyacrylamide Gel Female Juvenile Hormones/*physiology Male Moths/*physiology Neuropeptides/*isolation & purification/metabolism/physiology *Pheromones Protein Binding Up-Regulation/*physiology;" |
| Notes: | "MedlineRafaeli, A Zakharova, T Lapsker, Z Jurenka, R A eng Research Support, Non-U.S. Gov't England 2003/03/01 Insect Biochem Mol Biol. 2003 Mar; 33(3):371-80. doi: 10.1016/s0965-1748(02)00264-3" |
