| Title: | "Generation of arming yeasts with active proteins and peptides via cell surface display system: cell surface engineering, bio-arming technology" |
| Address: | "Laboratory of Biomacromolecular Chemistry, Division of Applied Life Sciences, Department of Applied Biochemistry, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake-cho, Sakyo-ku, Kyoto, 606-8502, Japan" |
| DOI: | 10.1007/978-1-4939-0563-8_8 |
| ISSN/ISBN: | 1940-6029 (Electronic) 1064-3745 (Linking) |
| Abstract: | "The cell surface display system in yeast enables the innovative strategy for improving cellular functions in a wide range of applications such as biofuel production, bioremediation, synthesis of valuable chemicals, recovery of rare metal ions, development of biosensors, and high-throughput screening of proteins/peptides library. Display of enzymes for polysaccharide degradation enables the construction of metabolically engineered whole-cell biocatalyst owing to the accessibility of the displayed enzymes to high-molecular-weight polysaccharides. In addition, along with fluorescence-based activity evaluation, fluorescence-activated cell sorting (FACS), and yeast cell chip, the cell surface display system is an effective molecular tool for high-throughput screening of mutated proteins/peptides library. In this article, we describe the methods for cell surface display of proteins/peptides of interest on yeast, evaluation of display efficiency, and harvesting of the displayed proteins/peptides from cell surface" |
| Keywords: | "Flow Cytometry Fluorescent Antibody Technique Genetic Engineering/*methods Mating Factor Peptides/*genetics/metabolism Plasmids/genetics Recombinant Proteins/*genetics Saccharomyces cerevisiae/*cytology/*genetics Transformation, Genetic;" |
| Notes: | "MedlineKuroda, Kouichi Ueda, Mitsuyoshi eng 2014/04/20 Methods Mol Biol. 2014; 1152:137-55. doi: 10.1007/978-1-4939-0563-8_8" |
