| Title: | 5'UTR methylation in different genes from workers exposed to volatile organic compounds: A new insight for considering an epigenetic mark as a functional correlate |
| Author(s): | Jimenez-Garza O; Linares-Segovia B; Ruiz-Garcia L; Monroy-Torres R; Hernandez-Luna MA; |
| Address: | "Health Sciences Division, University of Guanajuato, Leon Campus. Blvd. Puente del Milenio, 1001 Fracc. Del Predio San Carlos, CP 37670, Leon, Guanajuato, Mexico. Electronic address: ojimenezgarza@ugto.mx. Health Sciences Division, University of Guanajuato, Leon Campus. Blvd. Puente del Milenio, 1001 Fracc. Del Predio San Carlos, CP 37670, Leon, Guanajuato, Mexico" |
| DOI: | 10.1016/j.toxlet.2020.05.001 |
| ISSN/ISBN: | 1879-3169 (Electronic) 0378-4274 (Linking) |
| Abstract: | "Gene-specific methylation has been related with transcriptional/translational consequences in different cells; also, this epigenetic modification is affected by environmental exposures. In previous studies, CYP2E1 activity in toluene-exposed workers was decreased compared to controls, however, CYP2E1 promoter methylation levels did not show significant differences. Here, we compared gene-specific methylation levels at the 5'UTR region, in a subset of workers whom already participated in two former studies, compared to controls. METHODS: DNA was obtained from whole blood in five different groups: occupationally exposed to a mixture of volatile organic compounds (VOC): high levels (n = 19); low levels (n = 19) and very low levels (n = 17), toluene-exposed workers (n = 19) and control group (n = 19). We performed PCR-pyrosequencing at the 5'UTR region from four genes: CYP2E1, IL-6, SOD1 and TNF-alpha. RESULTS: In participants exposed to high levels of a VOC mixture, we found significant differences: lower methylation levels for IL-6, and higher methylation levels for TNF-alpha compared to controls. In toluene-exposed workers, we found significant, lower methylation levels for CYP2E1 compared to controls. CONCLUSION: Lower methylation levels at the 5'UTR region from CYP2E1 in toluene exposed-workers, suggests that this epigenetic modification could represent a functional correlate regarding enzymatic activity, as a response to toluene biotransformation" |
| Keywords: | 5'UTR region Cyp2e1 DNA methylation Epigenetic biomarkers Functional correlate Il6 Occupational exposure TNF-alpha VOC's; |
| Notes: | "PublisherJimenez-Garza, Octavio Linares-Segovia, Benigno Ruiz-Garcia, Liliana Monroy-Torres, Rebeca Hernandez-Luna, Marco Antonio eng Netherlands 2020/05/22 Toxicol Lett. 2020 May 11; 330:59-64. doi: 10.1016/j.toxlet.2020.05.001" |
