« Previous Abstract"CYP2E1 epigenetic regulation in chronic, low-level toluene exposure: Relationship with oxidative stress and smoking habit"    Next Abstract5'UTR methylation in different genes from workers exposed to volatile organic compounds: A new insight for considering an epigenetic mark as a functional correlate »

Toxicol Appl Pharmacol

Title:		Aberrant promoter methylation in genes related to hematopoietic malignancy in workers exposed to a VOC mixture
Author(s):		Jimenez-Garza O; Guo L; Byun HM; Carrieri M; Bartolucci GB; Barron-Vivanco BS; Baccarelli AA;
Address:		"Health Sciences Division, University of Guanajuato, Leon, Campus. Blvd. Puente del Mienio 1001, Fraccion del Predio San Carlos, C.P. 37670 Leon Guanajuato, Mexico. Electronic address: ojimenezgarza@ugto.mx. Department of Occupational & Environmental Health, Tianjin Medical University, No.22 Qixiangtai Road, Heping District, Tianjin 300070, China. Human Nutrition Research Centre, Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne NE4 5PL, United Kingdom. Department of Cardiologic, Thoracic and Vascular Science, University of Padova, Via Giustiniani, 2, 35128 Padova, Italy. The Laboratory of Environmental Toxicology and Pollution, Autonomous University of Nayarit, Av de la Cultura Amado Nervo S/N, CP 36000 Tepic, Nayarit, Mexico. The Laboratory of Human Environmental Epigenetics, Harvard T.H. Chan School of Public Health, 677 Huntington Avenue, Boston, MA 02115, USA"
Journal Title:		Toxicol Appl Pharmacol
Year:		2018
Volume:		20171205
Issue:
Page Number:		65 - 72
DOI:		10.1016/j.taap.2017.12.002
ISSN/ISBN:		1096-0333 (Electronic) 0041-008X (Linking)
Abstract:		"Occupational exposure to volatile organic compounds (VOCs) may cause hematopoietic malignancy, either by single exposure to benzene or possibly due to a concomitant exposure to several VOCs. Since oxidative stress, inflammation and DNA repair pathways are closely involved in cancer development, the effect of VOC exposure on expression of proteins involved in these pathways has been studied, but epigenetic changes have not been well described. Here, DNA methylation status following occupational exposure to a VOC mixture was assessed by bisulfite sequencing of the promoter regions of seven genes involved in the mentioned pathways. Peripheral blood samples and individual-level VOC exposure data were obtained from healthy leather shoe factory workers (LS, n=40) and gas station attendants (GS, n=36), as well as a reference group of university employees (C, n=66). Exposure levels for acetone, ethylbenzene, methyl ethyl ketone, n-hexane, toluene and xylene were higher in LS (p<0.001); benzene and methyl acetate levels were higher in GS (p<0.001). TOP2A, SOD1, and TNF-alpha promoter methylation status was increased in LS (p<0.05). In LS, we also found significant correlations between GSTP1 promoter methylation and both iNOS (r=0.37, p=0.008) and COX-2 (r=-0.38, p=0.007) methylation. In exposed groups, ethylbenzene exposure levels showed a significant correlation with TOP2A methylation (beta=0.33). Our results show early, toxic effects at the epigenetic level caused by occupational exposure to high levels of a VOC mixture. These subcellular modifications may represent the initial mechanism of toxicity leading to hematopoietic malignancy, possibly due to a synergistic, hematotoxic effect of VOC mixtures"
Keywords:		"Adolescent Adult Benzene Derivatives/toxicity DNA Methylation/*drug effects/physiology Female Hematologic Neoplasms/*blood/chemically induced/*genetics Humans Male Middle Aged Occupational Exposure/*adverse effects Promoter Regions, Genetic/*drug effects/;"
Notes:		"MedlineJimenez-Garza, Octavio Guo, Liqiong Byun, Hyang-Min Carrieri, Mariella Bartolucci, Giovanni Battista Barron-Vivanco, Briscia Socorro Baccarelli, Andrea A eng Research Support, Non-U.S. Gov't 2017/12/09 Toxicol Appl Pharmacol. 2018 Jan 15; 339:65-72. doi: 10.1016/j.taap.2017.12.002. Epub 2017 Dec 5"