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J Med Entomol

Title:		Oviposition responses of Anopheles gambiae s.s. (Diptera: Culicidae) and identification of volatiles from bacteria-containing solutions
Author(s):		Lindh JM; Kannaste A; Knols BG; Faye I; Borg-Karlson AK;
Address:		"Department of Genetics, Microbiology and Toxicology, Stockholm University, Stockholm S-10691, Sweden"
Journal Title:		J Med Entomol
Year:		2008
Volume:		45
Issue:		6
Page Number:		1039 - 1049
DOI:		10.1603/0022-2585(2008)45[1039:oroags]2.0.co;2
ISSN/ISBN:		0022-2585 (Print) 0022-2585 (Linking)
Abstract:		"In this study, a dual-choice oviposition bioassay was used to screen responses of gravid An. gambiae toward 17 bacterial species, previously isolated from Anopheles gambiae s.l. (Diptera: Culicidae) midguts or oviposition sites. The 10 isolates from oviposition sites have been identified by phylogenetic analyses of their 16S rRNA genes. Eight of the 10 isolates were gram-positive, out of which six belonged to the Bacilli class. Solid phase microextraction and gas chromatography coupled to mass spectrometry (GC-MS) were used to identify the volatiles emitted from the bacterial isolates. Aromatic and aliphatic alcohols, aliphatic ketones, alkylpyrazines, dimethyl oligosulfides, and indole were among the chemical compounds identified from the headspace above bacteria-containing saline. The mosquitoes laid significantly more eggs in six of the bacteria-containing solutions compared with the sterile solution. These six bacteria did not emit any compounds in common that could explain the positive oviposition response. Instead, the bacteria were grouped according to principal component analysis (PCA) based on the relative amounts of volatiles emitted. The PCA-plots facilitated the identification of 13 putative oviposition attractants for An. gambiae mosquitoes"
Keywords:		Animals Anopheles/microbiology/*physiology Bacteria/*chemistry/classification Female Gas Chromatography-Mass Spectrometry Gastrointestinal Tract/microbiology *Oviposition Pheromones/*analysis Principal Component Analysis Soil Microbiology Solid Phase Micr;
Notes:		"MedlineLindh, J M Kannaste, A Knols, B G J Faye, I Borg-Karlson, A K eng Research Support, Non-U.S. Gov't England 2008/12/09 J Med Entomol. 2008 Nov; 45(6):1039-49. doi: 10.1603/0022-2585(2008)45[1039:oroags]2.0.co; 2"