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J Vis Exp

Title:		A Step-by-Step Guide to Mosquito Electroantennography
Author(s):		Lahondere C;
Address:		"Department of Biochemistry; The Fralin Life Science Institute; The Global Change Center; Department of Entomology and the Center for Emerging Zoonotic and Arthropod-Borne Pathogens, Virginia Polytechnic Institute and State University; lahonder@vt.edu"
Journal Title:		J Vis Exp
Year:		2021
Volume:		20210310
Issue:		169
Page Number:		-
DOI:		10.3791/62042
ISSN/ISBN:		1940-087X (Electronic) 1940-087X (Linking)
Abstract:		"Female mosquitoes are the deadliest animals on earth, claiming the lives of more than 1 million people every year due to pathogens they transmit when acquiring a blood-meal. To locate a host to feed on, mosquitoes rely on a wide range of sensory cues, including visual, mechanical, thermal, and olfactory. The study details a technique, electroantennography (EAG), that allows researchers to assess whether the mosquitoes can detect individual chemicals and blends of chemicals in a concentration-dependent manner. When coupled with gas-chromatography (GC-EAG), this technique allows to expose the antennae to a full headspace/complex mixture and determines which chemicals present in the sample of interest, the mosquito can detect. This is applicable to host body odors as well as plant floral bouquets or other ecologically relevant odors (e.g., oviposition sites odorants). Here, we described a protocol that permits long durations of preparation responsiveness time and is applicable to both female and male mosquitoes from multiple genera, including Aedes, Culex, Anopheles, and Toxorhynchites mosquitoes. As olfaction plays a major part in mosquito-host interactions and mosquito biology in general, EAGs and GC-EAG can reveal compounds of interest for the development of new disease vector control strategies (e.g., baits). Complemented with behavioral assays, the valence (e.g., attractant, repellent) of each chemical can be determined"
Keywords:		Animals;Animals Biosensing Techniques/*instrumentation Mosquito Vectors;
Notes:		"MedlineLahondere, Chloe eng Research Support, Non-U.S. Gov't Video-Audio Media 2021/03/30 J Vis Exp. 2021 Mar 10; (169). doi: 10.3791/62042"