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Mol Cell Biol

Title:		Role of transcription factor Kar4 in regulating downstream events in the Saccharomyces cerevisiae pheromone response pathway
Author(s):		Lahav R; Gammie A; Tavazoie S; Rose MD;
Address:		"Department of Molecular Biology, Princeton University, Princeton, NJ 08544-1014, USA"
Journal Title:		Mol Cell Biol
Year:		2007
Volume:		20061113
Issue:		3
Page Number:		818 - 829
DOI:		10.1128/MCB.00439-06
ISSN/ISBN:		0270-7306 (Print) 1098-5549 (Electronic) 0270-7306 (Linking)
Abstract:		"Yeast Kar4 is a putative transcription factor required for karyogamy (the fusion of haploid nuclei during mating) and possibly other functions. Previously known to be required only for the transcriptional induction of KAR3 and CIK1, microarray experiments identified many genes regulated by Kar4 in both mating and mitosis. Several gene clusters are positively or negatively regulated by mating pheromone in a Kar4-dependent manner. Chromatin immunoprecipitation and gel shift assays confirmed that Kar4 binds to regulatory DNA sequences upstream of KAR3. Together with one-hybrid experiments, these data support a model in which both Kar4 and Ste12 bind jointly to the KAR3 promoter. Analysis of the upstream regions of Kar4-induced genes identified a DNA sequence motif that may be a binding site for Kar4. Mutation within the motif upstream of KAR3 eliminated pheromone induction. Genes regulated by Kar4, on average, are delayed in their temporal expression and exhibit a more stringent dose response to pheromone. Furthermore, the induction of Kar4 by pheromone is necessary for the delayed temporal induction of KAR3 and PRM2, genes required for efficient nuclear fusion during mating. Accordingly, we propose that Kar4 plays a critical role in the choreography of the mating response"
Keywords:		"Amino Acid Sequence Base Sequence Cluster Analysis Consensus Sequence DNA, Fungal/genetics/metabolism DNA-Binding Proteins/chemistry/genetics/*metabolism Gene Expression Regulation, Fungal/*drug effects Genes, Fungal Microtubule-Associated Proteins/geneti;"
Notes:		"MedlineLahav, Ron Gammie, Alison Tavazoie, Saeed Rose, Mark D eng R01 GM037739/GM/NIGMS NIH HHS/ GM 37739/GM/NIGMS NIH HHS/ Research Support, N.I.H., Extramural 2006/11/15 Mol Cell Biol. 2007 Feb; 27(3):818-29. doi: 10.1128/MCB.00439-06. Epub 2006 Nov 13"