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Environ Sci Pollut Res Int

Title:		Interactions between tannins allelochemicals and extracellular polymeric substance (EPS) of Microcystis aeruginosa
Author(s):		Yin L; Wang J; Shi K; Zhang Y; Xu Y; Kong D; Ni L; Li S;
Address:		"School of Environment, Nanjing Normal University, Nanjing, 210023, China. Department of Geological Sciences, University of Alabama, Tuscaloosa, AL, 35487, USA. Key Laboratory of Integrated Regulation and Resource Development On Shallow Lakes of Ministry of Education, School of Environment, Hohai University, Nanjing, 210098, China. School of Environment, Nanjing Normal University, Nanjing, 210023, China. lishiyin@njnu.edu.cn. Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and Application, Nanjing, 210023, China. lishiyin@njnu.edu.cn"
Journal Title:		Environ Sci Pollut Res Int
Year:		2022
Volume:		20220628
Issue:		55
Page Number:		83211 - 83219
DOI:		10.1007/s11356-022-21661-5
ISSN/ISBN:		1614-7499 (Electronic) 0944-1344 (Linking)
Abstract:		"The protective mechanism of extracellular polymeric substance (EPS) secreted by a harmful cyanobacteria against tannins allelochemicals was explored in this study. The binding properties of soluble EPS (SEPS) and bound EPS (BEPS) of Microcystis aeruginosa to tannic acid (TA) were investigated via fluorescence spectroscopy. The results suggested that TA interacted with the proteins in SEPS and BEPS mainly with binding constants of 5.26 and 7.93 L/mol, respectively; TA interacted with the humic acids in SEPS and BEPS mainly with binding constants of 5.12 and 5.24 L/mol, respectively. Thermodynamic experiments confirmed that the binding was mainly controlled by the hydrophobic force. Combined with Fourier transform infrared spectroscopy, it was found that the amine, carbonyl, carboxyl, and hydroxyl groups in EPS were the main functional groups contributing to the interaction of TA with EPS. The existence of EPS reduced the toxicity of TA to algal cells, with the 96 h inhibition rate of 40 mg L(-1) TA on algal cells decreasing by 48.95%. The results of this study may improve our understanding of the protective mechanism of cyanobacteria against tannins allelochemicals"
Keywords:		*Microcystis/metabolism Extracellular Polymeric Substance Matrix/chemistry Tannins Pheromones/pharmacology Humic Substances/analysis *Cyanobacteria Cyanobacteria Extracellular polymeric substance Interactions Microcystis aeruginosa Tannic acid;
Notes:		"MedlineYin, Li Wang, Juan Shi, Kaipian Zhang, Yong Xu, Ying Kong, Desheng Ni, Lixiao Li, Shiyin eng 51979137/National Natural Science Foundation of China/ 51779079/National Natural Science Foundation of China/ 41931292/National Natural Science Foundation of China/ Germany 2022/06/29 Environ Sci Pollut Res Int. 2022 Nov; 29(55):83211-83219. doi: 10.1007/s11356-022-21661-5. Epub 2022 Jun 28"