| Title: | Headspace measurements of irradiated in vitro cultured cells using PTR-MS |
| Author(s): | Brunner C; Szymczak W; Li W; Hoeschen C; Mortl S; Eckardt-Schupp F; Oeh U; |
| Address: | "Department of Medical Radiation Physics and Diagnostics, Helmholtz Zentrum Munchen, German Research Center for Environmental Health, Ingolstadter Landstrasse 1, 85764, Neuherberg, Germany. claudia.brunner@helmholtz-muenchen.de" |
| DOI: | 10.1007/s00411-010-0327-8 |
| ISSN/ISBN: | 1432-2099 (Electronic) 0301-634X (Linking) |
| Abstract: | "A pilot study was performed to evaluate a new concept for a radiation biodosimetry method. Proton transfer reaction-mass spectrometry (PTR-MS) was used to find out whether radiation induces changes in the composition of volatile organic compounds (VOCs) in the headspace of in vitro cultured cells. Two different cell lines, retinal pigment epithelium cells hTERT-RPE1 and lung epithelium cells A-549, were irradiated with gamma radiation at doses of 4 Gy and 8 Gy. For measuring the cell-specific effects, the VOC concentrations in the headspace of flasks containing cells plus medium, as well as of flasks containing pure medium were analyzed for changes before and after irradiation. No significant radiation-induced alterations in VOC concentrations in the headspace could be observed after irradiation" |
| Keywords: | Adsorption Cell Line Culture Media Epithelial Cells/*chemistry/*radiation effects Humans Mass Spectrometry/*methods *Protons Radiometry Time Factors Volatile Organic Compounds/chemistry Volatilization; |
| Notes: | "MedlineBrunner, C Szymczak, W Li, W Hoeschen, C Mortl, S Eckardt-Schupp, F Oeh, U eng Germany 2010/09/08 Radiat Environ Biophys. 2011 Mar; 50(1):209-17. doi: 10.1007/s00411-010-0327-8. Epub 2010 Sep 4" |
