« Previous AbstractOptimization and field demonstration of the Lygus pratensis (Hemiptera: Miridae) sex pheromone    Next AbstractVolatile organic compounds as potential biomarkers of irritable bowel syndrome: A systematic review »

Biochim Biophys Acta

Title:		"Structural characterization and transcriptional regulation of the gene encoding diapause hormone and pheromone biosynthesis activating neuropeptide in the cotton bollworm, Helicoverpa armigera"
Author(s):		Zhang TY; Sun JS; Liu WY; Kang L; Shen JL; Xu WH;
Address:		"Department of Molecular and Cell Biology, School of Life Sciences, University of Science and Technology of China, Hefei 230027, China"
Journal Title:		Biochim Biophys Acta
Year:		2005
Volume:		1728
Issue:		1-Feb
Page Number:		44 - 52
DOI:		10.1016/j.bbaexp.2005.01.009
ISSN/ISBN:		0006-3002 (Print) 0006-3002 (Linking)
Abstract:		"We have cloned the gene encoding the diapause hormone and the pheromone biosynthesis activating neuropeptide in Helicoverpa armigera (Har-DH-PBAN). The Har-DH-PBAN gene contains six exons and five introns that fall in the same positions as in the Bombyx mori DH-PBAN gene (Bom-DH-PBAN). The transcription initiation site lays 29 bp upstream of the translation initiation site. Southern blot analysis suggests that a single copy of this gene is present per haploid genome. A structural comparison of DH-PBAN promoters between H. armigera and B. mori show similarities in the TATA box and in a potential binding site for a POU family transcription factor, POU-M2. However, testing of these DNA regions for factor binding in vitro and transcription assays in cell culture highlight significant differences in their regulation particularly in reference to the POU-M2 sites. Our results uncover common and different regulatory mechanisms at work in the control of DH-PBAN gene expression in H. armigera and B. mori"
Keywords:		"Amino Acid Sequence Animals Base Sequence Blotting, Southern Cloning, Molecular DNA Primers Electrophoretic Mobility Shift Assay Gene Components *Gene Expression Regulation Genomic Library Luciferases Molecular Sequence Data Moths/*genetics Neuropeptides/;"
Notes:		"MedlineZhang, Tian-Yi Sun, Jiu-Song Liu, Wen-Yu Kang, Le Shen, Jin-Liang Xu, Wei-Hua eng Comparative Study Research Support, Non-U.S. Gov't Netherlands 2005/03/22 Biochim Biophys Acta. 2005 Apr 5; 1728(1-2):44-52. doi: 10.1016/j.bbaexp.2005.01.009"