| Title: | The temptin gene of the clade Lophotrochozoa is involved in formation of the prismatic layer during biomineralization in molluscs |
| Author(s): | Peng M; Liu Z; Li Z; Qian S; Liu X; Li J; |
| Address: | "Key Laboratory of Freshwater Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Agriculture, Shanghai 201306, China. Taizhou University, Jiangsu 225300, China. Electronic address: qianshanqin@163.com. Department of Biotechnology and Biomedicine, Yangtze Delta Region Institute of Tsinghua University, Zhejiang 314000, China. Electronic address: txliuxj@vip.163.com. Key Laboratory of Freshwater Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Agriculture, Shanghai 201306, China. Electronic address: jlli2009@126.com" |
| DOI: | 10.1016/j.ijbiomac.2021.07.164 |
| ISSN/ISBN: | 1879-0003 (Electronic) 0141-8130 (Linking) |
| Abstract: | "The biomineralization mechanism of mollusc shell has been studied for a long time, but there is a lack of understanding about the relationship between the shell formation in vitro and the signaling system in vivo. In this study, we cloned a novel shell matrix protein gene (hc-temptin), which only be characterized as a water-borne protein pheromone of molluscs in previous studies, from the freshwater mussel Hyriopsis cumingii. By bioinformatics analysis we found that temptin was a gene unique to the clade Lophotrochozoa, and it exists in all mollusc taxa except Cephalopoda. The current data supported the premise that temptin was generated in the early emergence of molluscs and that it maintained a high mutation rate to evolve relative independently. The specificity of hc-temptin expression in the mantle tissue suggests its potential to participate in biomineralization. Its sequence contained typical Ca(2+) binding sites. Our experiments involving the pearl formation process, damaged shell repair process, and RNAi experiment showed that hc-temptin was a shell matrix protein that plays an important role in formation of the prismatic layer. The results of this study provided new insights about the origin of the temptin gene and its role in molluscs" |
| Keywords: | "Amino Acid Sequence Animals Base Sequence Biomineralization/*genetics DNA, Complementary/genetics Evolution, Molecular Gene Expression Profiling Gene Expression Regulation Gene Silencing Nacre/metabolism *Phylogeny Proteins/chemistry/*genetics/metabolism;" |
| Notes: | "MedlinePeng, Maoxiao Liu, Zhenming Li, Zhi Qian, Shanqin Liu, Xiaojun Li, Jiale eng Netherlands 2021/08/03 Int J Biol Macromol. 2021 Oct 1; 188:800-810. doi: 10.1016/j.ijbiomac.2021.07.164. Epub 2021 Jul 31" |
