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New Phytol

Title:		"The dynamics of cereal cyst nematode infection differ between susceptible and resistant barley cultivars and lead to changes in (1,3;1,4)-beta-glucan levels and HvCslF gene transcript abundance"
Author(s):		Aditya J; Lewis J; Shirley NJ; Tan HT; Henderson M; Fincher GB; Burton RA; Mather DE; Tucker MR;
Address:		"Australian Research Council Centre of Excellence in Plant Cell Walls and School of Agriculture, Food and Wine, Waite Research Institute, The University of Adelaide, PMB 1, Glen Osmond, SA, 5064, Australia. South Australian Research and Development Institute, GPO Box 397, Adelaide, SA, 5001, Australia"
Journal Title:		New Phytol
Year:		2015
Volume:		20150303
Issue:		1
Page Number:		135 - 147
DOI:		10.1111/nph.13349
ISSN/ISBN:		1469-8137 (Electronic) 0028-646X (Linking)
Abstract:		"Heterodera avenae (cereal cyst nematode, CCN) infects the roots of barley (Hordeum vulgare) forming syncytial feeding sites. In resistant host plants, relatively few females develop to maturity. Little is known about the physiological and biochemical changes induced during CCN infection. Responses to CCN infection were investigated in resistant (Rha2) and susceptible barley cultivars through histological, compositional and transcriptional analysis. Two phases were identified that influence CCN viability, including feeding site establishment and subsequent cyst maturation. Syncytial development progressed faster in the resistant cultivar Chebec than in the susceptible cultivar Skiff, and was accompanied by changes in cell wall polysaccharide abundance, particularly (1,3;1,4)-beta-glucan. Transcriptional profiling identified several glycosyl transferase genes, including CELLULOSE SYNTHASE-LIKE F10 (HvCslF10), which may contribute to differences in polysaccharide abundance between resistant and susceptible cultivars. In barley, Rha2-mediated CCN resistance drives rapid deterioration of CCN feeding sites, specific changes in cell wall-related transcript abundance and changes in cell wall composition. During H. avenae infection, (1,3;1,4)-beta-glucan may influence CCN feeding site development by limiting solute flow, similar to (1,3)-beta-glucan during dicot cyst nematode infections. Dynamic transcriptional changes in uncharacterized HvCslF genes, possibly involved in (1,3;1,4)-beta-glucan synthesis, suggest a role for these genes in the CCN infection process"
Keywords:		"Animals Cell Wall Cluster Analysis Crosses, Genetic Disease Resistance/*genetics Disease Susceptibility Fluorescence Gene Expression Regulation, Plant Genes, Plant Giant Cells/physiology Herbivory/physiology Hordeum/*genetics/immunology/*parasitology Mono;"
Notes:		"MedlineAditya, Jessika Lewis, John Shirley, Neil J Tan, Hwei-Ting Henderson, Marilyn Fincher, Geoffrey B Burton, Rachel A Mather, Diane E Tucker, Matthew R eng Research Support, Non-U.S. Gov't England 2015/03/05 New Phytol. 2015 Jul; 207(1):135-147. doi: 10.1111/nph.13349. Epub 2015 Mar 3"