| Title: | Characterization of medium chain length (R)-3-hydroxycarboxylic acids produced by Streptomyces sp. JM3 and the evaluation of their antimicrobial properties |
| Author(s): | Allen AD; Daley P; Ayorinde FO; Gugssa A; Anderson WA; Eribo BE; |
| Address: | "Department of Biology, Howard University, 415 College St. NW, Washington, DC 20059, USA. adriandallen@yahoo.com" |
| Journal Title: | World J Microbiol Biotechnol |
| DOI: | 10.1007/s11274-012-1089-z |
| ISSN/ISBN: | 1573-0972 (Electronic) 0959-3993 (Linking) |
| Abstract: | "(R)-3-Hydroxycarboxylic acids, chiral enantiomers of bacterial polyhydroxyalkanoates (PHA), may be valuable synthons for the production of numerous industrial materials such as beta-lactams, fungicides, flavors, pheromones and vitamins. In this study, (R)-3-hydroxycarboxylic acid [(R)-3HAs)] synthons were produced by Streptomyces sp. JM3 (JN166713) under batch fermentation. Initial confirmation of PHA production was achieved by matrix assisted laser desorption ionization-time of flight mass spectroscopy and gas chromatography/mass spectroscopy (GC/MS). Subsequently, (R)-3HAs were produced by in vivo depolymerization and the monomers were separated using acid precipitation and anion exchange chromatography. The (R)-3HAs were identified by GC/MS as 3-trimethylsiloxy esters of decanoic, octanoic and butanoic acids. This was further supported by (13)C nuclear magnetic resonance spectrometry. The (R)-3HAs exhibited antimicrobial activity against Escherichia coli O157:H7, Listeria monocytogenes (ATCC 7644) and Salmonella typhimurium (ATCC 14028) with minimum inhibitory concentration ranging from 12.5 to 25 mg ml(-1). However, the minimum bactericidal concentration data suggest that the (R)-3HAs may be bactericidal for E. coli O157:H7 and bacteriostatic for S. typhimurium and L. monocytogenes. Furthermore, the major purified synthon was shown to minimize the invasion of fibroblasts by S. typhimurium (ATCC 14028) [p < 0.05], using the MTT assay [(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)]" |
| Keywords: | "3T3 Cells Animals Anti-Infective Agents/*analysis/*pharmacology Colony Count, Microbial Escherichia coli O157/drug effects Fermentation Fibroblasts/microbiology Food Microbiology Food Preservation/methods Gas Chromatography-Mass Spectrometry Listeria mono;" |
| Notes: | "MedlineAllen, Adrian Douglas Daley, Patrick Ayorinde, Folahan O Gugssa, Ayelle Anderson, Winston A Eribo, Broderick E eng Howard Hughes Medical Institute/ Research Support, Non-U.S. Gov't Germany 2012/07/19 World J Microbiol Biotechnol. 2012 Sep; 28(9):2791-800. doi: 10.1007/s11274-012-1089-z. Epub 2012 Jun 19" |
