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Mol Syst Biol

Title:		Fus3-triggered Tec1 degradation modulates mating transcriptional output during the pheromone response
Author(s):		Chou S; Zhao S; Song Y; Liu H; Nie Q;
Address:		"Department of Biological Chemistry, University of California, Irvine, CA 92697, USA"
Journal Title:		Mol Syst Biol
Year:		2008
Volume:		20080805
Issue:
Page Number:		212 -
DOI:		10.1038/msb.2008.47
ISSN/ISBN:		1744-4292 (Electronic) 1744-4292 (Linking)
Abstract:		"The yeast transcription factor Ste12 controls both mating and filamentation pathways. Upon pheromone induction, the mitogen-activated protein kinases, Fus3 and Kss1, activate Ste12 by relieving the repression of two functionally redundant Ste12 inhibitors, Dig1 and Dig2. Mating genes are controlled by the Ste12/Dig1/Dig2 complex through Ste12-binding sites, whereas filamentation genes are regulated by the Tec1/Ste12/Dig1 complex through Tec1-binding sites. The two Ste12 complexes are mutually exclusive. During pheromone response, Tec1 is degraded upon phosphorylation by Fus3, preventing cross-activation of the filamentation pathway. Here, we show that a stable Tec1 also impairs the induction of mating genes. A mathematical model is developed to capture the dynamic formation of the two Ste12 complexes and their interactions with pathway-specific promoters. By model simulations and experimentation, we show that excess Tec1 can impair the mating transcriptional output because of its ability to sequester Ste12, and because of a novel function of Dig2 for the transcription of mating genes. We suggest that Fus3-triggered Tec1 degradation is an important part of the transcriptional induction of mating genes during the pheromone response"
Keywords:		"Computer Simulation DNA-Binding Proteins/genetics/*metabolism *Gene Expression Regulation, Fungal Mitogen-Activated Protein Kinases/genetics/*metabolism Models, Genetic Mutation Phosphorylation Promoter Regions, Genetic Reproduction Saccharomyces cerevisi;"
Notes:		"MedlineChou, Song Zhao, Su Song, You Liu, Haoping Nie, Qing eng R01GM67247/GM/NIGMS NIH HHS/ R01 GM067247/GM/NIGMS NIH HHS/ P50GM76516/GM/NIGMS NIH HHS/ P50 GM076516/GM/NIGMS NIH HHS/ R01GM/AI55155/AI/NIAID NIH HHS/ R01GM75309/GM/NIGMS NIH HHS/ R01 GM075309/GM/NIGMS NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't England 2008/08/07 Mol Syst Biol. 2008; 4:212. doi: 10.1038/msb.2008.47. Epub 2008 Aug 5"