| Title: | Fus3-regulated Tec1 degradation through SCFCdc4 determines MAPK signaling specificity during mating in yeast |
| Address: | "Department of Biological Chemistry, University of California, Irvine, Irvine, CA 92697, USA" |
| DOI: | 10.1016/j.cell.2004.11.053 |
| ISSN/ISBN: | 0092-8674 (Print) 0092-8674 (Linking) |
| Abstract: | "Signaling specificity is fundamental for parallel mitogen-activated protein kinase (MAPK) cascades that control growth and differentiation in response to different stimuli. In Saccharomyces cerevisiae, components of the pheromone-responsive MAPK cascade activate Fus3 and Kss1 MAPKs to induce mating and Kss1 to promote filamentation. Active Fus3 is required to prevent the activation of the filamentation program during pheromone response. How Fus3 prevents the crossactivation is not clear. Here we show that Tec1, a cofactor of Ste12 for the expression of filamentation genes, is rapidly degraded during pheromone response. Fus3 but not Kss1 induces Tec1 ubiquination and degradation through the SCFCdc4 ubiquitin ligase. T273 in a predicted high-affinity Cdc4 binding motif is phosphorylated by Fus3 both in vitro and in vivo. Tec1T273V blocks Tec1 ubiquitination and degradation and allows the induction of filamentation genes in response to pheromone. Thus, Fus3 inhibits filamentous growth during mating by degrading Tec1" |
| Keywords: | "Cell Cycle Proteins/genetics/*metabolism DNA-Binding Proteins/genetics/*metabolism F-Box Proteins/genetics/*metabolism Gene Expression Regulation, Fungal *MAP Kinase Signaling System/drug effects Mating Factor Mitogen-Activated Protein Kinases/genetics/*m;" |
| Notes: | "MedlineChou, Song Huang, Lan Liu, Haoping eng R01 GM055155/GM/NIGMS NIH HHS/ GM55155/GM/NIGMS NIH HHS/ Research Support, U.S. Gov't, P.H.S. 2004/12/29 Cell. 2004 Dec 29; 119(7):981-90. doi: 10.1016/j.cell.2004.11.053" |
