| Title: | Chemical vapor detection using a reconstituted insect olfactory receptor complex |
| Address: | "Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo (Japan); ERATO Takeuchi Biohybrid Innovation Project, JST, Komaba Open Laboratory, 4-6-1 Komaba, Meguro-ku, Tokyo (Japan)" |
| ISSN/ISBN: | 1521-3773 (Electronic) 1433-7851 (Linking) |
| Abstract: | "The sensing of vapor odorants is highly demanded in the field of life and medical sciences. Although olfactory receptors (ORs) have potentials to recognize volatile organic compounds, the interaction of ORs, chemical vapors, and peptide components in olfactory mucus has yet to be analyzed to develop OR-based sensors. A bioinspired electrophysiology technique is shown to record the response of reconstituted insect ORs to chemical vapors. To mimic the interface between ORs and olfactory mucus, OR expressing spheroids were loaded into a hydrogel microchamber array. A negative extracellular field potential shift of spheroids was successfully observed by the stimulation of their vapor cognate ligand. Importantly, the ligand repertoire of the OR of malaria vector mosquito examined by this method differed from that of in vivo studies. Our method is useful to develop protein-based gas sensing techniques and to examine the binding of ORs and chemical vapors" |
| Keywords: | "Animals Biosensing Techniques/*methods Insecta Receptors, Odorant/*chemistry Volatile Organic Compounds/*chemistry biosensors ion channels olfaction receptors;" |
| Notes: | "MedlineSato, Koji Takeuchi, Shoji eng Research Support, Non-U.S. Gov't Germany 2014/07/30 Angew Chem Int Ed Engl. 2014 Oct 27; 53(44):11798-802. doi: 10.1002/anie.201404720. Epub 2014 Jul 28" |
