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Clin Chim Acta

Title:		Increased breath biomarkers of oxidative stress in diabetes mellitus
Author(s):		Phillips M; Cataneo RN; Cheema T; Greenberg J;
Address:		"Menssana Research Inc., 1 Horizon Road, Suite 1415, Fort Lee, NJ 07024, USA. mphillips@menssanaresearch.com"
Journal Title:		Clin Chim Acta
Year:		2004
Volume:		344
Issue:		1-Feb
Page Number:		189 - 194
DOI:		10.1016/j.cccn.2004.02.025
ISSN/ISBN:		0009-8981 (Print) 0009-8981 (Linking)
Abstract:		"BACKGROUND: Oxidative stress has been implicated in the major complications of diabetes mellitus, including retinopathy, nephropathy, neuropathy and accelerated coronary artery disease. There is a clinical need for a marker of oxidative stress which could potentially identify diabetic patients at increased risk for these complications. We measured oxidative age, a new breath marker of oxidative stress, in diabetic patients. METHODS: Three groups were studied: type 1 diabetes mellitus (n=9), type 2 diabetes mellitus (n=53) and non-diabetic normals (n=39). Volatile organic compounds (VOCs) in breath were assayed by gas chromatography and mass spectroscopy to construct the breath methylated alkane contour (BMAC), a three-dimensional display of oxidative stress markers, C4-C20 alkanes and monomethylated alkanes. The collective abundance of these VOCs was reduced to a single value, the oxidative age, comprising the volume under the curve of the BMAC corrected for chronological age. RESULTS: Oxidative age was significantly increased in type 1 diabetes (mean=0.103, S.E.M.=0.119, p<0.01) and type 2 diabetes (mean=0.103, S.E.M.=0.047, p<0.05) compared to age-matched normals (mean=-0.248, S.E.M.=0.079). No significant correlation between oxidative age and blood glucose or hemoglobin A1C was observed in either group. CONCLUSIONS: Oxidative age, a marker of oxidative stress, was significantly increased in both type 1 and type 2 diabetes mellitus. Oxidative age merits further study as a candidate marker of risk for the complications of diabetes mellitus"
Keywords:		"Adult Alkanes/analysis Biomarkers/analysis Breath Tests Case-Control Studies Diabetes Complications/*diagnosis/metabolism Diabetes Mellitus/*metabolism Diabetes Mellitus, Type 1/metabolism Diabetes Mellitus, Type 2/metabolism Gas Chromatography-Mass Spect;"
Notes:		"MedlinePhillips, Michael Cataneo, Renee N Cheema, Taseer Greenberg, Joel eng Netherlands 2004/05/20 Clin Chim Acta. 2004 Jun; 344(1-2):189-94. doi: 10.1016/j.cccn.2004.02.025"