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Methods Cell Biol

Title:		Methods for evaluating the Caenorhabditis elegans dauer state: standard dauer-formation assay using synthetic daumones and proteomic analysis of O-GlcNAc modifications
Author(s):		Lee J; Kim KY; Joo HJ; Kim H; Jeong PY; Paik YK;
Address:		"Yonsei Proteome Research Center, Department of Biochemistry and Biomedical Science, College of Life Science and Biotechnology, World Class University Program, Graduate School, Yonsei University, Seoul, Korea"
Journal Title:		Methods Cell Biol
Year:		2011
Volume:		106
Issue:
Page Number:		445 - 460
DOI:		10.1016/B978-0-12-544172-8.00016-5
ISSN/ISBN:		0091-679X (Print) 0091-679X (Linking)
Abstract:		"The dauer state is a non-feeding, alternative L3 state characterized by a number of distinctive metabolic and morphological changes. There are many naturally occurring dauer-inducing pheromones, termed daumones, that have been suggested by some to exhibit differences in dauer-inducing activity. Here, we have established a standard dauer-formation assay that uses synthetic daumones 1, 2, and 3, the three major daumones. To analyze the proteome of Caenorhabditis elegans in the dauer state, we focused on O-GlcNAc modification, a cytosolic modification of proteins that is known to interact either competitively or synergistically with protein phosphorylation. Protein O-GlcNAc modification is an important biological process in cells that can ensure the timely response to extracellular stimuli, such as daumone, and maintain cellular homeostasis. Establishing a standard method for assaying dauer formation using different synthetic daumones, and using differences in O-GlcNAcylated proteins during the dauer state to analyze the dauer proteome will lead to a better understanding of dauer biology of C. elegans in the context of animal longevity and adaptation under harsh environments"
Keywords:		"Animals Caenorhabditis elegans/metabolism/*physiology Caenorhabditis elegans Proteins/chemistry/*metabolism Culture Techniques Electrophoresis, Gel, Two-Dimensional Fatty Acids/*metabolism/pharmacology/physiology Glycosylation Pheromones/*metabolism/pharm;"
Notes:		"MedlineLee, Jeeyong Kim, Kwang-Youl Joo, Hyoe-Jin Kim, Heekyeong Jeong, Pan-Young Paik, Young-Ki eng Research Support, Non-U.S. Gov't Review 2011/11/29 Methods Cell Biol. 2011; 106:445-60. doi: 10.1016/B978-0-12-544172-8.00016-5"