| Title: | Study on Bombykol Receptor Self-Assembly and Universality of G Protein Cellular Signal Amplification System |
| Address: | "College of Biotechnology & Food Science , Tianjin University of Commerce , Tianjin 300134 , China. Tianjin Key Laboratory of Food Biotechnology , Tianjin 300134 , China. College of Biotechnology , Tianjin University of Science &Technology , Tianjin 300457 , China. Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education , Tianjin 300457 , China" |
| DOI: | 10.1021/acssensors.8b01446 |
| ISSN/ISBN: | 2379-3694 (Electronic) 2379-3694 (Linking) |
| Abstract: | "The G protein cascade amplification system couples with several receptors to sense/amplify the cellular signal, implying universal application. In order to explore whether GPCRs can trigger G protein signal amplification in tissues/cells from different species, bombykol receptor was isolated and purified from antennas of male Bombyx mori, which subsequently self-assembled on the cell membrane in rat taste buds/rat vomeronasa/catfish tentacles/taste bud tissues of rabbits/pig/cattle in those lacking endogenous bombykol receptor, followed by immobilization between two sheets of nucleopore membranes fixed by sodium alginate-starch gel, forming the sandwich-type sensing membrane, which in turn was immobilized on the glass-carbon electrode. Thus, bombykol receptor sensors were established with different tissues. The response current of bombykol receptor sensor toward bombykol was measured with an electrochemical workstation. Every bombykol receptor sensor could sense bombykol based on enzyme-substrate kinetics. The double reciprocal plot and the activation constant values of bombykol receptor sensors assembled with rat taste buds, rat vomeronasa, catfish tentacles, rabbit taste buds, pig taste buds, and cattle taste buds were calculated. Approximately 2-3 receptors could trigger the G protein cascade amplification system and achieve the maximum signal output. Moreover, the detection lower limit indicated that the bombykol receptor self-assembled on the cell membranes of different tissues that transmitted and amplified the bombykol signal with hypersensitivity. Also, cattle taste bud tissues served as an ideal system for heterogeneous GPCRs self-assembly and signal sensing/amplification. This sensing technique and method had promising potential in studies of biological pest control, sex pheromone detection, and receptor structure and function" |
| Keywords: | "Animals Biosensing Techniques/methods Bombyx/chemistry Catfishes Cattle Cell Membrane/metabolism Fatty Alcohols/*analysis/metabolism Insect Proteins/*metabolism Limit of Detection Male Protein Multimerization Rabbits Rats Receptors, Pheromone/*metabolism;" |
| Notes: | "MedlineXu, Qiuda Lu, Dingqiang Pang, Guangchang eng Research Support, Non-U.S. Gov't 2019/01/09 ACS Sens. 2019 Jan 25; 4(1):257-264. doi: 10.1021/acssensors.8b01446. Epub 2019 Jan 15" |
