| Title: | Plant allelochemicals affect tolerance of polyphagous lepidopteran pest Helicoverpa armigera (Hubner) against insecticides |
| Author(s): | Chen S; Elzaki MEA; Ding C; Li ZF; Wang J; Zeng RS; Song YY; |
| Address: | "College of Natural Resources and Environment, South China Agricultural University, Wushan, Guangzhou 510642, China; College of Materials and Energy, South China Agricultural University, Wushan, Guangzhou 510642, China. Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Crop Science, Fujian Agriculture and Forestry University, Jinshan, Fuzhou 350002, China. College of Natural Resources and Environment, South China Agricultural University, Wushan, Guangzhou 510642, China. Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Crop Science, Fujian Agriculture and Forestry University, Jinshan, Fuzhou 350002, China. Electronic address: rszeng@fafu.edu.cn. Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Crop Science, Fujian Agriculture and Forestry University, Jinshan, Fuzhou 350002, China. Electronic address: yyuansong@163.com" |
| DOI: | 10.1016/j.pestbp.2018.12.009 |
| ISSN/ISBN: | 1095-9939 (Electronic) 0048-3575 (Linking) |
| Abstract: | "Cotton bollworm, Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae), is a polyphagous lepidopteran pest distributed worldwide with a broad spectrum of host plants. However, the mechanism of H. armigera adaptation to various insecticides and defensive allelochemicals in its host plants is not fully understood. Therefore, this study examined the influence of consumption of plant allelochemicals on larval tolerance to methomyl and chlorpyrifos insecticides in H. armigera and its possible mechanism. Twelve plant allelochemicals were screened to evaluate their effects on larval sensitivity to methomyl. Of which flavone, coumarin, DIMBOA (2,4-Dihydroxy-7-methoxy-1,4-benzoxazin-3-one) and visnagin significantly reduced larval sensitivity to methomyl. Application of cytochrome P450 inhibitor piperonyl butoxide (PBO) significantly increased the mortality of methomyl-treated larvae. In contrast, PBO addition significantly decreased the mortality of chlorpyrifos-treated larvae. Moreover, allelochemical consumption enhanced the activities of glutathione S-transferase, carboxylesterase, cytochrome P450 and acetylcholinesterase in the midgut and fat body. The qRT-PCR analysis confirms that P450 genes, CYP6B2, CYP6B6 and CYP6B7 were induced by the four allelochemicals in the midguts and the fat bodies. In conclusion, the generalist H. armigera can take benefit of plant allelochemicals from its host plants to elaborate its defense against insecticides" |
| Keywords: | "Animals Carboxylesterase/genetics Chlorpyrifos/toxicity Cytochrome P-450 Enzyme System/genetics Female Gene Expression Regulation, Enzymologic/drug effects Glutathione Transferase/genetics Insect Proteins/genetics Insecticide Resistance/*drug effects/gene;" |
| Notes: | "MedlineChen, Shi Elzaki, Mohammed Esmail Abdalla Ding, Chaohui Li, Zheng-Fang Wang, Jie Zeng, Ren-Sen Song, Yuan-Yuan eng 2019/02/16 Pestic Biochem Physiol. 2019 Feb; 154:32-38. doi: 10.1016/j.pestbp.2018.12.009. Epub 2018 Dec 18" |
