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J Chem Ecol

Title:		Ammonia Released by Streptomyces aburaviensis Induces Droplet Formation in Streptomyces violaceoruber
Author(s):		Schmidt K; Spiteller D;
Address:		"Chemical Ecology/Biological Chemistry, Department of Biology, University of Konstanz, Universitatstrasse 10, 78457, Konstanz, Germany. Chemical Ecology/Biological Chemistry, Department of Biology, University of Konstanz, Universitatstrasse 10, 78457, Konstanz, Germany. dieter.spiteller@uni-konstanz.de"
Journal Title:		J Chem Ecol
Year:		2017
Volume:		20170722
Issue:		8
Page Number:		806 - 816
DOI:		10.1007/s10886-017-0870-8
ISSN/ISBN:		1573-1561 (Electronic) 0098-0331 (Linking)
Abstract:		"Streptomyces violaceoruber grown in co-culture with Streptomyces aburaviensis produces an about 17-fold higher volume of droplets on its aerial mycelium than in single-culture. Physical separation of the Streptomyces strains by either a plastic barrier or by a dialysis membrane, which allowed communication only by the exchange of volatile compounds or diffusible compounds in the medium, respectively, still resulted in enhanced droplet formation. The application of molecular sieves to bioassays resulted in the attenuation of the droplet-inducing effect of S. aburaviensis indicating the absorption of the compound. (1)H-NMR analysis of molecular-sieve extracts and the selective indophenol-blue reaction revealed that the volatile droplet-inducing compound is ammonia. The external supply of ammonia in biologically relevant concentrations of >/=8 mM enhanced droplet formation in S. violaceoruber in a similar way to S. aburaviensis. Ammonia appears to trigger droplet production in many Streptomyces strains because four out of six Streptomyces strains exposed to ammonia exhibited induced droplet production"
Keywords:		Ammonia/analysis/chemistry/*metabolism Biological Assay Diffusion Gases/chemistry Hydrogen-Ion Concentration Lipid Droplets/*chemistry Magnetic Resonance Spectroscopy Organic Chemicals/chemistry Streptomyces/*chemistry/growth & development/metabolism Alka;
Notes:		"MedlineSchmidt, Kathrin Spiteller, Dieter eng 2017/07/25 J Chem Ecol. 2017 Aug; 43(8):806-816. doi: 10.1007/s10886-017-0870-8. Epub 2017 Jul 22"