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J Immunol Methods

Title:		The use of polyclonal activators in the production of murine monoclonal and polyclonal antibodies
Author(s):		Fiori PL; Rappelli P;
Address:		"Istituto di Microbiologia e Virologia, Universita degli Studi di Sassari, Italy"
Journal Title:		J Immunol Methods
Year:		1991
Volume:		139
Issue:		2
Page Number:		181 - 190
DOI:		10.1016/0022-1759(91)90187-k
ISSN/ISBN:		0022-1759 (Print) 0022-1759 (Linking)
Abstract:		We propose a new immunization method to stimulate a strong immune response against weak or diluted antigens. This technique is based on stimulation with polyclonal activators before exposure to the antigens. We also discuss the efficiency of various types of mitogen with particular regard to their capacity to produce monoclonal antibodies and serum antibodies. A specific immune response against soluble antigens is increased by pretreating mice with PPD. This preactivation permitted us to obtain monoclonal antibodies against weak antigens in a few days. No monoclonal antibodies were obtained by inoculating weak antigens or the activators by themselves
Keywords:		"*Adjuvants, Immunologic Animals Antibodies, Bacterial/immunology Antibodies, Monoclonal/*immunology Arginase/immunology Conalbumin/immunology Cytoskeleton/immunology Hybridomas/*immunology Immunization/*methods Immunization Schedule *Lymphocyte Activation;"
Notes:		"MedlineFiori, P L Rappelli, P eng Research Support, Non-U.S. Gov't Netherlands 1991/06/03 J Immunol Methods. 1991 Jun 3; 139(2):181-90. doi: 10.1016/0022-1759(91)90187-k"