| Title: | Specific and common epitopes in mating pheromones of Euplotes raikovi revealed by monoclonal antibodies |
| Author(s): | Fiori PL; Miceli C; Raffioni S; Vallesi A; |
| Address: | "Institute of Microbiology and Virology, University of Sassari, Italy" |
| DOI: | 10.1111/j.1550-7408.1990.tb01125.x |
| ISSN/ISBN: | 0022-3921 (Print) 0022-3921 (Linking) |
| Abstract: | "Polypeptide mating pheromones Er-1 and Er-2, purified from the supernatant of Euplotes raikovi cultures of mating type I and mating type II, respectively, were used to immunize mice and obtain monoclonal antibodies. Five hybridoma clones producing antibodies specific to the mating pheromones were selected. They were analyzed for immunospecificity by immunoperoxidase assay, immunoblotting, and for their efficacy in inhibition of mating pheromone activity. Monoclonal antibodies from two hybridoma clones recognized only the mating pheromone used as antigen: those from the other three clones reacted, to comparable extents, with both mating pheromones. On the basis of these results it was assumed that two immunogenic sites exist in Er-1 and Er-2, one specific and the other common to both mating pheromones" |
| Keywords: | "Animals Antibodies, Monoclonal/biosynthesis/immunology Antibodies, Protozoan/biosynthesis/immunology Antibody Specificity Ciliophora/*immunology Epitopes/*immunology Hybridomas Immunoenzyme Techniques Immunoglobulin Isotypes/immunology Mice Mice, Inbred B;" |
| Notes: | "MedlineFiori, P L Miceli, C Raffioni, S Vallesi, A eng Research Support, Non-U.S. Gov't 1990/05/01 J Protozool. 1990 May-Jun; 37(3):187-90. doi: 10.1111/j.1550-7408.1990.tb01125.x" |
