| Title: | Pheromone-induced morphogenesis improves osmoadaptation capacity by activating the HOG MAPK pathway |
| Author(s): | Baltanas R; Bush A; Couto A; Durrieu L; Hohmann S; Colman-Lerner A; |
| Address: | "Instituto de Fisiologia, Biologia Molecular y Neurociencias, Consejo Nacional de Investigaciones Cientificas y Tecnicas y Departamento de Fisiologia, Biologia Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires C1428EHA, Argentina" |
| DOI: | 10.1126/scisignal.2003312 |
| ISSN/ISBN: | 1937-9145 (Electronic) 1945-0877 (Linking) |
| Abstract: | "Environmental and internal conditions expose cells to a multiplicity of stimuli whose consequences are difficult to predict. We investigate the response to mating pheromone of yeast cells adapted to high osmolarity. Events downstream of pheromone binding involve two mitogen-activated protein kinase (MAPK) cascades: the pheromone response (PR) and the cell wall integrity (CWI) response. Although the PR MAPK pathway shares components with a third MAPK pathway, the high osmolarity (HOG) response, each one is normally only activated by its cognate stimulus, a phenomenon called insulation. We found that in cells adapted to high osmolarity, PR activated the HOG pathway in a pheromone- and osmolarity-dependent manner. Activation of HOG by the PR was not due to loss of insulation, but rather a response to a reduction in internal osmolarity, which resulted from an increase in glycerol release caused by the PR. By analyzing single-cell time courses, we found that stimulation of HOG occurred in discrete bursts that coincided with the 'shmooing' morphogenetic process. Activation required the polarisome, the CWI MAPK Slt2, and the aquaglyceroporin Fps1. HOG activation resulted in high glycerol turnover, which improved adaptability to rapid changes in osmolarity. Our work shows how a differentiation signal can recruit a second, unrelated sensory pathway to fine-tune yeast response in a complex environment" |
| Keywords: | Cell Wall/genetics/metabolism MAP Kinase Signaling System/*physiology Membrane Proteins/genetics/*metabolism Mitogen-Activated Protein Kinases/genetics/*metabolism Osmosis/physiology Pheromones/genetics/*metabolism Saccharomyces cerevisiae/cytology/geneti; |
| Notes: | "MedlineBaltanas, Rodrigo Bush, Alan Couto, Alicia Durrieu, Lucia Hohmann, Stefan Colman-Lerner, Alejandro eng R01 GM097479/GM/NIGMS NIH HHS/ 1R01GM097479-01/GM/NIGMS NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't 2013/04/25 Sci Signal. 2013 Apr 23; 6(272):ra26. doi: 10.1126/scisignal.2003312" |
