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Eur J Biochem

Title:		The oligosaccharides of the glycoprotein pheromone of Volvox carteri f. nagariensis Iyengar (Chlorophyceae)
Author(s):		Balshusemann D; Jaenicke L;
Address:		"Institute of Biochemistry, Universitat zu Koln, Federal Republic of Germany"
Journal Title:		Eur J Biochem
Year:		1990
Volume:		192
Issue:		1
Page Number:		231 - 237
DOI:		10.1111/j.1432-1033.1990.tb19220.x
ISSN/ISBN:		0014-2956 (Print) 0014-2956 (Linking)
Abstract:		"The sexuality-inducing glycoprotein of Volvox carteri f. nagariensis was purified from supernatants of disintegrated sperm packets of the male strain IPS-22 and separated by reverse-phase HPLC into several isoforms which differ in the degree of O-glycosylation. Total chemical deglycosylation with trifluoromethanesulphonic acid yields the biologically inactive core protein of 22.5 kDa. This core protein possesses three putative binding sites for N-glycans which are clustered in the middle of the polypeptide chain. The N-glycosidically bound oligosaccharides were obtained by glycopeptidase F digestion and were shown by a combination of exoglycosidase digestion, gaschromatographic sugar analysis and two-dimensional HPLC separation to possess the following definite structures: (A) Man beta 1-4GlcNAc beta 1-4GlcNAc; (B) (Man alpha)3 Man beta 1-4GlcNAc beta 1-4GlcNAc Xyl beta; (C) (Man alpha)2 Man beta 1-4GlcNAc beta 1-4GlcNAc; (D) (Man)2Xyl(GlcNAc)2. Xyl beta Two of the three N-glycosidic binding sites carry one B and one D glycan. The A and C glycans are shared by the third N-glycosylation site. The O-glycosidic sugars, which make up 50% of the total carbohydrate, are short (up to three sugar residues) chains composed of Ara, Gal and Xyl and are exclusively bound to Thr residues"
Keywords:		"Carbohydrate Sequence Chlorophyta/*analysis Chromatography, Gas Electrophoresis, Polyacrylamide Gel/methods Glycoproteins/*isolation & purification Molecular Sequence Data Monosaccharides *Oligosaccharides Peptides Pheromones/*isolation & purification;"
Notes:		"MedlineBalshusemann, D Jaenicke, L eng Research Support, Non-U.S. Gov't England 1990/08/28 Eur J Biochem. 1990 Aug 28; 192(1):231-7. doi: 10.1111/j.1432-1033.1990.tb19220.x"