| Title: | Time-Course of CYP450 Genes Expression From Dendroctonus rhizophagus (Curculionidae: Scolytinae) During Early Hours of Drilling Bark and Settling Into the Host Tree |
| Author(s): | Sarabia LE; Lopez MF; Pineda-Mendoza RM; Obregon-Molina G; Gonzalez-Escobedo R; Albores-Medina A; Zuniga G; |
| Address: | "Laboratorio de Variacion Biologica y Evolucion, Departamento de Zoologia, Escuela Nacional de Ciencias Biologicas, Instituto Politecnico Nacional, Prolongacion de Carpio y Plan de Ayala s/n, Miguel Hidalgo, Mexico City, Mexico. Departamento de Toxicologia, Centro de Investigacion y de Estudios Avanzados del Instituto Politecnico Nacional, Av. Instituto Politecnico Nacional 2508, San Pedro Zacatenco, Gustavo A. Madero, Mexico City, Mexico" |
| ISSN/ISBN: | 1536-2442 (Electronic) 1536-2442 (Linking) |
| Abstract: | "Dendroctonus bark beetles (Scolytinae) are one of the most important disturbance agents of coniferous forests in North and Central America. These beetles spend their lives almost entirely under the tree bark, and their survival and reproductive success depend on their ability to overcome the toxic effect of the trees' oleoresin. The cytochromes P450 (CYPs) are associated with the detoxification process of xenobiotics, as well as other physiological processes. Different cytochromes (families 4, 6, and 9) in the Dendroctonus species have been expressed under several experimental conditions; nevertheless, the expression time-course of these genes is unknown. To explore the induction speed of CYPs, we evaluated the relative expression of the CYP6BW5, CYP6DG1, CYP6DJ2, CYP9Z18, and CYP9Z20 genes at the early hours of drilling and settling into a tree (1, 2, 4, 6, 8, 12, 18 h) both in females and males, solitary or paired, of the bark beetle Dendroctonus rhizophagus Thomas and Bright. Our findings show that the five genes were rapidly overexpressed in the early hours (1 to 6 h) in both sexes and in solitary and paired conditions, suggesting their participation in the detoxification process. Additionally, the CYPs expression shows up- and down-regulation patterns through these short times, suggesting their probable participation in other physiological processes as the biosynthesis of hormones, pheromones or compounds related to reproduction" |
| Keywords: | Animals Cytochrome P-450 Enzyme System/genetics/*metabolism Feeding Behavior Female Male Trees Weevils/*enzymology/genetics Cyp450 bark beetle detoxification monoterpene; |
| Notes: | "MedlineSarabia, Laura E Lopez, Maria F Pineda-Mendoza, Rosa M Obregon-Molina, Gabriel Gonzalez-Escobedo, Roman Albores-Medina, Arnulfo Zuniga, Gerardo eng 2019/05/23 J Insect Sci. 2019 May 1; 19(3):11. doi: 10.1093/jisesa/iez046" |
