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Pak J Pharm Sci

Title:		Volatile constituents and in vitro activity of Syzygium aromaticum flower buds (clove) against human cancer cell lines
Author(s):		Sara; Begum S; Ali SN; Farooq AD; Siddiqui F; Siddiqui BS; Ayub A; Fatima M;
Address:		"HEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Pakistan/Department of Chemistry University of Karachi, Karachi, Pakistan. HEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Pakistan. Department of Chemistry University of Karachi, Karachi, Pakistan. Hamdard Al-Majeed College of Eastern Medicine Hamdard University, Karachi, Pakistan. Faculty of Pharmaceutical Sciences, Dow College of Pharmacy, Dow University of Health Sciences, Karachi, Pakistan. HEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Pakistan/ NED University of Engineering & Technology, Karachi, Pakistan"
Journal Title:		Pak J Pharm Sci
Year:		2020
Volume:		33
Issue:		6
Page Number:		2659 - 2665
DOI:
ISSN/ISBN:		1011-601X (Print) 1011-601X (Linking)
Abstract:		"The methanolic extract (SA-EXT) of Syzygium aromaticum flower buds and its fractions tested against three human cancer cell lines viz uterine cervix (HeLa), breast (MCF-7) and lung NCI (H-460) using sulforhodamine-B assay. The ethyl acetate soluble sub fraction (SA-EAS) was active only against HeLa cells with GI(50)value of 36+/- 3.4mug/mL. The most active sub-fraction (SA-PES-Fr-2) showed growth inhibition (GI(50): 36, 50 and 68mug/ml against MCF-7, HeLa and NCI-H-460 cancer cell lines, respectively) with cytotoxic effect LC(50)= 88 +/- 3.4 mug/mL against HeLa and LC(50)=86 +/- 2.8 mug/mL against MCF-7. The most active sub-fraction (SA-PES-Fr-2) analyzed by GC and GC-MS techniques revealed that eugenol was most abundant (85.34%) along with minor constituents. Thus it can be concluded that growth inhibitory and cytotoxic effect residing in Syzygium aromaticum flower buds (clove) is more likely due to eugenol"
Keywords:		"Acetates/chemistry Antineoplastic Agents, Phytogenic/*chemistry/*pharmacology Cell Line, Tumor Drug Screening Assays, Antitumor Eugenol/analysis/pharmacology Flowers/chemistry Gas Chromatography-Mass Spectrometry HeLa Cells Humans MCF-7 Cells Methanol/che;"
Notes:		"MedlineSara, - Begum, Sabira Ali, Syed Nawazish Farooq, Ahsana Dar Siddiqui, Faheema Siddiqui, Bina S Ayub, Anjum Fatima, Mashhad eng Pakistan 2021/04/20 Pak J Pharm Sci. 2020 Nov; 33(6):2659-2665"