| Title: | Synthesis and biological activity of a photoaffinity-biotinylated pheromone-biosynthesis activating neuropeptide (PBAN) analog |
| Address: | "Department of Stored Products, Volcani Center, Bet Dagan, Israel. vtada@volcani.agri.gov.il" |
| DOI: | 10.1016/s0196-9781(99)00063-7 |
| ISSN/ISBN: | 0196-9781 (Print) 0196-9781 (Linking) |
| Abstract: | "To study the mode of action of pheromone-biosynthesis activating neuropeptide (PBAN) at the receptor level and for receptor purification, we synthesized and tested the biologic properties of a photoaffinity biotinylated PBAN analog N-[N-(4-azido-tetrafluorobenzoyl)-biocytinyloxyl-succinimide (Atf-Bct-NHS-PBAN). The Atf-Bct-NHS-PBAN was separated from unreacted reagent and synthetic Hez-PBAN by high-performance liquid chromatography. Conjugated biotin was detected by using enzyme-linked assay as well as tricine sodium dodecyl sulfate polyacrylamide gel electrophoresis. The biologic activity of purified Atf-Bct-NHS-PBAN was confirmed using both in vivo and in vitro pheromonotropic bioassays. These observations indicate that Atf-Bct-NHS-PBAN is a full agonist of PBAN action in pheromone glands and may be used to study PBAN receptors by employing avidin coupled to various reporter groups" |
| Keywords: | "Affinity Labels/*chemical synthesis Animals Avidin Biological Assay Biotin Female In Vitro Techniques Male Moths Neuropeptides/*chemical synthesis/*pharmacology Pheromones/*biosynthesis Receptors, Neuropeptide/drug effects;" |
| Notes: | "MedlineRafaeli, A Gileadi, C eng Research Support, Non-U.S. Gov't 1999/09/07 Peptides. 1999; 20(7):787-94. doi: 10.1016/s0196-9781(99)00063-7" |
