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Rapid Commun Mass Spectrom


Title:Solid-phase microextraction gas chromatography/mass spectrometry: a new method for species identification of truffles
Author(s):Gioacchini AM; Menotta M; Bertini L; Rossi I; Zeppa S; Zambonelli A; Piccoli G; Stocchi V;
Address:"Istituto di Ricerca sull'Attivita Motoria, Universita degli Studi di Urbino, via Sasso 75/G, 61029 Urbino PU, Italy. a.gioacchini@uniurb.it"
Journal Title:Rapid Commun Mass Spectrom
Year:2005
Volume:19
Issue:17
Page Number:2365 - 2370
DOI: 10.1002/rcm.2031
ISSN/ISBN:0951-4198 (Print) 0951-4198 (Linking)
Abstract:"This study describes a rapid method to identify different truffle species by analysis of their volatile compound fraction using static headspace solid-phase microextraction gas chromatography/mass spectrometry. The volatile organic compounds (VOCs) were extracted using a new 2-cm 50/30 microm DVB/CAR/PDMS fiber placed for 10 min in the headspace of the truffle sample with the vial maintained at 20 degrees C (in a thermostatically controlled analysis room). The mass spectra of the VOC chromatograms were represented as 'fingerprints' of the analysed samples. Next, stepwise factorial discriminant analysis afforded a limited number of characteristic fragment ions that allowed a classification of the truffle species studied. This new method provides an effective approach to rapid quality control and identification of truffle species by analysis of their volatile fraction. Moreover, this method offers the advantage of minimizing thermal, mechanical, and chemical modifications of the truffles, thereby reducing the risk of analytical artifacts"
Keywords:Fungi/*chemistry/*classification/growth & development Gas Chromatography-Mass Spectrometry/*methods Species Specificity Time Factors;
Notes:"MedlineGioacchini, Anna Maria Menotta, Michele Bertini, Luana Rossi, Ismaela Zeppa, Sabrina Zambonelli, Alessandra Piccoli, Giovanni Stocchi, Vilberto eng England 2005/07/28 Rapid Commun Mass Spectrom. 2005; 19(17):2365-70. doi: 10.1002/rcm.2031"

 
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