« Previous Abstract"Enantiomer and conformer recognition of (+) and (-)-disparlure and their analogs by the pheromone binding proteins of the gypsy moth, Lymantria dispar"    Next Abstract"Differential field responses of the little fire ant, Wasmannia auropunctata (Roger), to alarm pheromone enantiomers" »

Nucleic Acids Res

Title:		Histone variant Htz1 promotes histone H3 acetylation to enhance nucleotide excision repair in Htz1 nucleosomes
Author(s):		Yu Y; Deng Y; Reed SH; Millar CB; Waters R;
Address:		"Institute of Cancer & Genetics, School of Medicine, Cardiff University, Cardiff CF14 4XN, UK and Faculty of Life Sciences, Michael Smith Building, University of Manchester, Manchester M13 9PT, UK"
Journal Title:		Nucleic Acids Res
Year:		2013
Volume:		20130807
Issue:		19
Page Number:		9006 - 9019
DOI:		10.1093/nar/gkt688
ISSN/ISBN:		1362-4962 (Electronic) 0305-1048 (Print) 0305-1048 (Linking)
Abstract:		"Nucleotide excision repair (NER) is critical for maintaining genome integrity. How chromatin dynamics are regulated to facilitate this process in chromatin is still under exploration. We show here that a histone H2A variant, Htz1 (H2A.Z), in nucleosomes has a positive function in promoting efficient NER in yeast. Htz1 inherently enhances the occupancy of the histone acetyltransferase Gcn5 on chromatin to promote histone H3 acetylation after UV irradiation. Consequently, this results in an increased binding of a NER protein, Rad14, to damaged DNA. Cells without Htz1 show increased UV sensitivity and defective removal of UV-induced DNA damage in the Htz1-bearing nucleosomes at the repressed MFA2 promoter, but not in the HMRa locus where Htz1 is normally absent. Thus, the effect of Htz1 on NER is specifically relevant to its presence in chromatin within a damaged region. The chromatin accessibility to micrococcal nuclease in the MFA2 promoter is unaffected by HTZ1 deletion. Acetylation on previously identified lysines of Htz1 plays little role in NER or cell survival after UV. In summary, we have identified a novel aspect of chromatin that regulates efficient NER, and we provide a model for how Htz1 influences NER in Htz1 nucleosomes"
Keywords:		Acetylation Adenosine Triphosphatases/genetics Chromatin/chemistry DNA Damage *DNA Repair DNA Repair Enzymes/metabolism Gene Deletion Histone Acetyltransferases/metabolism Histones/genetics/*metabolism Lipoproteins/genetics Microbial Viability Nucleosomes;
Notes:		"MedlineYu, Yachuan Deng, Yanbo Reed, Simon H Millar, Catherine B Waters, Raymond eng WT_/Wellcome Trust/United Kingdom 12340/CRUK_/Cancer Research UK/United Kingdom G0400113/MRC_/Medical Research Council/United Kingdom MR/K000926/1/MRC_/Medical Research Council/United Kingdom G0301154/MRC_/Medical Research Council/United Kingdom Research Support, Non-U.S. Gov't England 2013/08/09 Nucleic Acids Res. 2013 Oct; 41(19):9006-19. doi: 10.1093/nar/gkt688. Epub 2013 Aug 7"