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Bioorg Med Chem

Title:		"Enantiomer and conformer recognition of (+) and (-)-disparlure and their analogs by the pheromone binding proteins of the gypsy moth, Lymantria dispar"
Author(s):		Yu Y; Plettner E;
Address:		"Department of Chemistry, Simon Fraser University, 8888 University Drive, Burnaby, British Columbia, Canada"
Journal Title:		Bioorg Med Chem
Year:		2013
Volume:		20130131
Issue:		7
Page Number:		1811 - 1822
DOI:		10.1016/j.bmc.2013.01.043
ISSN/ISBN:		1464-3391 (Electronic) 0968-0896 (Linking)
Abstract:		"Adult female gypsy moths produce a sex pheromone (+)-(7R,8S)-2-methyl-7,8-epoxyoctadecane, (+)-disparlure, to attract male gypsy moths. To better understand the recognition of (+)-disparlure by the male's olfactory system, we synthesized racemic and enantiopure oxa and thia analogs of (+)-disparlure (ee>98%). Ab initio calculations of the conformeric landscapes around the dihedral angles C5-6-7-8 and C7-8-9-10 of (+)-disparlure and corresponding dihedral angles of analogs revealed that introduction of the heteroatom changes the conformeric landscape around these important epitopes. The energy difference between HOMO and LUMO decreased after oxygen or sulfur was introduced into the backbone. Consistent with this, an enhancement of binding affinity between sulfur analogs and the pheromone-binding proteins (PBPs) was observed in vitro. Docking of the pheromone and analogs onto models of the two known PBPs of the gypsy moth revealed that the internal binding pocket of PBP1 showed higher selectivity than that of PBP2, consistent with in vitro binding assays. Further energy analysis revealed that enantiomers adopted different conformations with different energies when docked in the internal binding pocket of PBPs, resulting in enantiomer discrimination of PBPs towards disparlure and its analogs"
Keywords:		Alkanes/chemistry/*metabolism Animals Binding Sites Female Insect Proteins/chemistry/*metabolism Male Molecular Conformation Molecular Docking Simulation Moths/chemistry/*metabolism Pheromones/chemistry/*metabolism Protein Binding Stereoisomerism Thermody;
Notes:		"MedlineYu, Yang Plettner, Erika eng Research Support, Non-U.S. Gov't England 2013/02/26 Bioorg Med Chem. 2013 Apr 1; 21(7):1811-22. doi: 10.1016/j.bmc.2013.01.043. Epub 2013 Jan 31"